BRCA1 Is Required for Meiotic Spindle Assembly and Spindle Assembly Checkpoint Activation in Mouse Oocytes

• Published in: Biology of reproduction Vol. 79; no. 4; pp. 718 - 726
• Main Authors: Xiong, Bo, Li, Sen, Ai, Jun-Shu, Yin, Shen, OuYang, Ying-Chun, Sun, Shao-Chen, Chen, Da-Yuan, Sun, Qing-Yuan
• Format: Journal Article
• Language: English
• Published: Madison, WI Society for the Study of Reproduction, Inc 01.10.2008; Society for the Study of Reproduction
• Subjects: Aneuploidy; Animals; Biological and medical sciences; BRCA1 Protein - metabolism; BRCA1 Protein - physiology; Cell Cycle Proteins - metabolism; Cells, Cultured; Embryo, Mammalian; Female; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Mad2 Proteins; Mammalian female genital system; Meiosis - drug effects; Meiosis - physiology; Mice; Nocodazole - pharmacology; Nuclear Proteins - metabolism; Oocytes - drug effects; Oocytes - metabolism; Oocytes - physiology; Oogenesis - drug effects; Oogenesis - physiology; Paclitaxel - pharmacology; Spindle Apparatus - drug effects; Spindle Apparatus - metabolism; Tissue Distribution - drug effects; Tubulin Modulators - pharmacology; Vertebrates: reproduction; Chromosomal aberration; Checkpointing; Embryo; spindle assembly; spindle assembly checkpoint; Rodentia; Aneuploidy; Breast cancer; Meiosis; Germinal cell; aneuploid embryo; mouse oocyte; Mitotic spindle; In vitro; Vertebrata; Mammalia; Mouse; BRCA 1; Oocyte; Tumor suppressor gene

BRCA1 as a tumor suppressor has been widely investigated in mitosis, but its functions in meiosis are unclear. In the present study, we examined the expression, localization, and function of BRCA1 during mouse oocyte meiotic maturation. We found that expression level of BRCA1 was increased progressi...