Effects of ingesting Lactobacillus- and Bifidobacterium-containing yogurt in subjects with colonized Helicobacter pylori

• Published in: The American journal of clinical nutrition Vol. 80; no. 3; pp. 737 - 741
• Main Authors: Wang, K.Y, Li, S.N, Liu, C.S, Perng, D.S, Su, Y.C, Wu, D.C, Jan, C.M, Lai, C.H, Wang, T.N, Wang, W.M
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Clinical Nutrition 01.09.2004; American Society for Clinical Nutrition, Inc
• Subjects: Adult; Antibiosis; Bacteria; Bifidobacterium; Bifidobacterium - physiology; Bifidobacterium animalis; Biological and medical sciences; Breath Tests; Carbon Isotopes; Colony Count, Microbial; diagnostic use; Feeding. Feeding behavior; Female; Food Microbiology; Fundamental and applied biological sciences. Psychology; growth & development; Helicobacter Infections; Helicobacter Infections - microbiology; Helicobacter Infections - prevention & control; Helicobacter pylori; Helicobacter pylori - growth & development; Humans; Infections; lactic acid bacteria; Lactobacillus; Lactobacillus - physiology; Lactobacillus acidophilus; Lactobacillus acidophilus - physiology; Male; microbial load; microbiology; Middle Aged; milk; mixed culture; physiology; placebos; prevention & control; Probiotics; therapeutics; Treatment Outcome; urease; Vertebrates: anatomy and physiology, studies on body, several organs or systems; viability; volunteers; Yogurt; Yogurt - microbiology; Human; Probiotic; Bifidobacterium; Actinomycetaceae; Spirillales; Lactobacillus acidophilus La5; Spirillaceae; Yogurt; Lactobacillus acidophilus; Actinomycetales; Urea; Probiotics; Breath test; Helicobacter pylori; Bifidobacterium lactis Bb12; Bacteria; Lactobacillaceae; Actinomycetes; urea breath test
• DOI: 10.1093/ajcn/80.3.737
• ISSN: 0002-9165

Background: Evidence suggests that ingesting lactic acid bacteria exerts a suppressive effect on Helicobacter pylori infection in both animals and humans. Supplementing with Lactobacillus- and Bifidobacterium-containing yogurt (AB-yogurt) was shown to improve the rates of eradication of H. pylori in humans. Objective: We administered AB-yogurt to subjects with asymptomatic H. pylori to test whether the yogurt could inhibit H. pylori growth. Design: The in vitro inhibition of H. pylori growth was determined by inoculating Lactobacillus acidophilus La5 or Bifidobacterium lactis Bb12 on plates that were inoculated with H. pylori. Assessment of the viability of H. pylori was performed by the mixed culture method with La5 or Bb12. In an intervention study, 59 adult volunteers infected with H. pylori were given AB-yogurt (10(7) colony-forming units of both La5 and Bb12/mL) twice daily after a meal for 6 wk. Eleven subjects positive for H. pylori infection were treated with milk placebo as control subjects. H. pylori bacterial loads were determined with use of the 13C-urea breath test, which was performed before and 4 and 8 wk after the start of AB-yogurt supplementation. Results: Bb12 exerted an in vitro inhibitory effect against H. pylori, whereas La5 did not show an effect. Administration of AB-yogurt decreased the urease activity of H. pylori after 6 wk of therapy (P < 0.0001). Conclusion: Regular intake of yogurt containing Bb12 and La5 effectively suppressed H. pylori infection in humans.