Maintenance of bovine oocytes in meiotic arrest and subsequent development in vitro: a comparative evaluation of antral follicle culture with other methods

• Published in: Biology of reproduction Vol. 59; no. 2; pp. 255 - 262
• Main Authors: Fouladi Nashta, A.A. (Roslin Institute, Roslin, Midlothian, Scotland, UK.), Waddington, D, Campbell, K.H.S
• Format: Journal Article
• Language: English
• Published: Madison, WI Society for the Study of Reproduction 01.08.1998
• Subjects: Animals; Biological and medical sciences; CATTLE; Cell Count; Cell Cycle - physiology; Cell Survival - physiology; DEVELOPMENTAL COMPETENCE; EMBRYO CULTURE; EMBRYONIC DEVELOPMENT; Female; FERTILIZATION; Fertilization in Vitro; Fundamental and applied biological sciences. Psychology; GAMETES; GERMINAL VESICLE; IN VITRO CULTURE; IN VITRO FERTILIZATION; IN VITRO MATURATION; Ionophores - pharmacology; Mammalian female genital system; MATURATION; MEIOSIS; Meiosis - physiology; Morphology. Physiology; Okadaic Acid - pharmacology; OOCYTE MATURATION; Oocytes - growth & development; Organ Culture Techniques; OVA; Ovarian Follicle - cytology; OVARIAN FOLLICLES; Vertebrates: reproduction; Cell culture; Ox; Germinal vesicle; Germinal cell; In vitro; Ovary; Vertebrata; Mammalia; Investigation method; Embryo culture; Female genital system; Artiodactyla; Ovarian follicle; Ungulata; Oocyte; Oocyte maturation

The frequency of development of bovine embryos produced by maturation, fertilization, and culture in vitro is lower than that observed in vivo. One factor that may affect both the frequency of development and the quality of the embryos produced is the developmental competence of the oocyte. In curre...