Mutation in the catalytic subunit of DNA polymerase α influences transcriptional gene silencing and homologous recombination in Arabidopsis
REPRESSOR OF SILENCING 1 (ROS1) encodes a DNA demethylase that actively removes DNA methylation. Mutation in ROS1 leads to transcriptional gene silencing of a T-DNA locus that contains two genes, RD29A-LUC and 35S-NPTII, originally expressed in the C24 wild type. These units have different silencing...
Saved in:
Published in | The Plant journal : for cell and molecular biology Vol. 61; no. 1; pp. 36 - 45 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Oxford, UK : Blackwell Publishing Ltd
2010
Blackwell Publishing Ltd Blackwell |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | REPRESSOR OF SILENCING 1 (ROS1) encodes a DNA demethylase that actively removes DNA methylation. Mutation in ROS1 leads to transcriptional gene silencing of a T-DNA locus that contains two genes, RD29A-LUC and 35S-NPTII, originally expressed in the C24 wild type. These units have different silencing regulation mechanisms: the former mechanism is dependent on small interfering RNA (siRNA)-directed DNA methylation, but the latter is not. We studied the latter gene silencing mechanism by screening the suppressors of the ros1 mutant using the silenced 35S-NPTII as a selection marker gene. The polα/incurvata2 (icu2) gene was isolated as one ros1 suppressor because its mutation leads to the reactivation of the silenced 35S-NPTII gene. POLα/ICU2 encodes a catalytic subunit of DNA polymerase α. Mutation of POLα/ICU2 did not affect DNA methylation, but reduced histone H3 Lys9 dimethylation (H3K9me2) modification in the 35S promoter. The polα mutation also influences the development of the shoot apical meristem, and delays the G2/M phase with high expression of a G2/M marker gene CycB1;1:GUS. Furthermore, the frequency of homologous recombination is greater in the polα/icu2 mutant than in the C24 wild type. Our results suggest that DNA polymerase α is involved in mediating epigenetic states and in DNA homologous recombination in Arabidopsis. |
---|---|
AbstractList | Summary
REPRESSOR OF SILENCING 1 (ROS1) encodes a DNA demethylase that actively removes DNA methylation. Mutation in ROS1 leads to transcriptional gene silencing of a T‐DNA locus that contains two genes, RD29A‐LUC and 35S‐NPTII, originally expressed in the C24 wild type. These units have different silencing regulation mechanisms: the former mechanism is dependent on small interfering RNA (siRNA)‐directed DNA methylation, but the latter is not. We studied the latter gene silencing mechanism by screening the suppressors of the ros1 mutant using the silenced 35S‐NPTII as a selection marker gene. The polα/incurvata2 (icu2) gene was isolated as one ros1 suppressor because its mutation leads to the reactivation of the silenced 35S‐NPTII gene. POLα/ICU2 encodes a catalytic subunit of DNA polymerase α. Mutation of POLα/ICU2 did not affect DNA methylation, but reduced histone H3 Lys9 dimethylation (H3K9me2) modification in the 35S promoter. The polα mutation also influences the development of the shoot apical meristem, and delays the G2/M phase with high expression of a G2/M marker gene CycB1;1:GUS. Furthermore, the frequency of homologous recombination is greater in the polα/icu2 mutant than in the C24 wild type. Our results suggest that DNA polymerase α is involved in mediating epigenetic states and in DNA homologous recombination in Arabidopsis. REPRESSOR OF SILENCING 1 (ROS1) encodes a DNA demethylase that actively removes DNA methylation. Mutation in ROS1 leads to transcriptional gene silencing of a T-DNA locus that contains two genes, RD29A-LUC and 35S-NPTII, originally expressed in the C24 wild type. These units have different silencing regulation mechanisms: the former mechanism is dependent on small interfering RNA (siRNA)-directed DNA methylation, but the latter is not. We studied the latter gene silencing mechanism by screening the suppressors of the ros1 mutant using the silenced 35S-NPTII as a selection marker gene. The polα/incurvata2 (icu2) gene was isolated as one ros1 suppressor because its mutation leads to the reactivation of the silenced 35S-NPTII gene. POLα/ICU2 encodes a catalytic subunit of DNA polymerase α. Mutation of POLα/ICU2 did not affect DNA methylation, but reduced histone H3 Lys9 dimethylation (H3K9me2) modification in the 35S promoter. The polα mutation also influences the development of the shoot apical meristem, and delays the G2/M phase with high expression of a G2/M marker gene CycB1;1:GUS. Furthermore, the frequency of homologous recombination is greater in the polα/icu2 mutant than in the C24 wild type. Our results suggest that DNA polymerase α is involved in mediating epigenetic states and in DNA homologous recombination in Arabidopsis. REPRESSOR OF SILENCING 1 (ROS1) encodes a DNA demethylase that actively removes DNA methylation. Mutation in ROS1 leads to transcriptional gene silencing of a T-DNA locus that contains two genes, RD29A-LUC and 35S-NPTII, originally expressed in the C24 wild type. These units have different silencing regulation mechanisms: the former mechanism is dependent on small interfering RNA (siRNA)-directed DNA methylation, but the latter is not. We studied the latter gene silencing mechanism by screening the suppressors of the ros1 mutant using the silenced 35S-NPTII as a selection marker gene. The polalpha/incurvata2 (icu2) gene was isolated as one ros1 suppressor because its mutation leads to the reactivation of the silenced 35S-NPTII gene. POLalpha/ICU2 encodes a catalytic subunit of DNA polymerase alpha. Mutation of POLalpha/ICU2 did not affect DNA methylation, but reduced histone H3 Lys9 dimethylation (H3K9me2) modification in the 35S promoter. The polalpha mutation also influences the development of the shoot apical meristem, and delays the G2/M phase with high expression of a G2/M marker gene CycB1;1:GUS. Furthermore, the frequency of homologous recombination is greater in the polalpha/icu2 mutant than in the C24 wild type. Our results suggest that DNA polymerase alpha is involved in mediating epigenetic states and in DNA homologous recombination in Arabidopsis. |
Author | Wang, Yu Yin, Haibo Ren, Xiaozhi Wang, Youqun Gong, Zhizhong Xia, Ran Liu, Jun |
Author_xml | – sequence: 1 fullname: Liu, Jun – sequence: 2 fullname: Ren, Xiaozhi – sequence: 3 fullname: Yin, Haibo – sequence: 4 fullname: Wang, Yu – sequence: 5 fullname: Xia, Ran – sequence: 6 fullname: Wang, Youqun – sequence: 7 fullname: Gong, Zhizhong |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22259791$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/19769574$$D View this record in MEDLINE/PubMed |
BookMark | eNpFkctu1DAUhi3Uik4LrwDesEzwJbHjBYtRC6VVuUi0EjvrxHGmHiV2FCei8w68TF-EZ6rDlMEbWzrfOUe_v1N05IO3CGFKcprO-21OuSgzTvnPnBGiclIQJvKHF2h1KByhFVGCZLKg7ASdxrglhEouipfohCopVCmLFfr9ZZ5gcsFj5_F0b7GBCbrd5AyOcz17N-HQ4ouvazyEbtfbEaLFfx4T3Xaz9cZGPI3goxndsIyBDm-stzi6LlWd32DwDb4PfejCJswRj9aEvnb-sHQ9Qu2aMEQXX6HjFrpoXz_fZ-ju08fb88_ZzbfLq_P1TdaySoiM1pIpKUtlqahkxaWRKQu1FdjaCKGAQV1YkI0iqjHcMFnXUDaSlIpXspX8DL3Zzx3mureNHkbXw7jT_74lAe-eAYgGujYlNC4eOMZYqaSiifuw536luLv_c4heNOmtXmzoxYZeNOm_mvSDvv1-vbxS_9t9fwtBw2ZMO-5-MEJ5MsWKkir-BEMplIQ |
ContentType | Journal Article |
Copyright | 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd 2015 INIST-CNRS |
Copyright_xml | – notice: 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd – notice: 2015 INIST-CNRS |
DBID | FBQ IQODW CGR CUY CVF ECM EIF NPM |
DOI | 10.1111/j.1365-313X.2009.04026.x |
DatabaseName | AGRIS Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Botany |
EISSN | 1365-313X |
EndPage | 45 |
ExternalDocumentID | 19769574 22259791 TPJ4026 US201301724519 |
Genre | article Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- -DZ .3N .GA .Y3 05W 0R~ 10A 123 1OC 24P 29O 2WC 31~ 33P 36B 3SF 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52U 52W 52X 53G 5HH 5LA 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A03 AAESR AAEVG AAHHS AANLZ AAONW AASGY AAXRX AAZKR ABCQN ABCUV ABEML ABHUG ABJNI ABPTK ABPVW ABWRO ACAHQ ACCFJ ACCZN ACFBH ACGFS ACIWK ACNCT ACPOU ACPRK ACSCC ACXBN ACXME ACXQS ADAWD ADBBV ADDAD ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN AEEZP AEGXH AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFEBI AFFPM AFGKR AFPWT AFRAH AFVGU AFZJQ AGJLS AIURR AIWBW AJBDE AJXKR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ATUGU AUFTA AZBYB AZVAB BAFTC BAWUL BFHJK BHBCM BMNLL BMXJE BNHUX BROTX BRXPI BY8 C45 CAG COF CS3 D-E D-F DCZOG DIK DPXWK DR2 DRFUL DRSTM DU5 E3Z EBS ECGQY EJD ESX F00 F01 F04 F5P FBQ FIJ G-S G.N GODZA H.T H.X HF~ HZI HZ~ IHE IPNFZ IX1 J0M K48 LATKE LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRSTM MSFUL MSSTM MXFUL MXSTM N04 N05 N9A NF~ O66 O9- OK1 OVD P2P P2W P2X P4D PQQKQ Q.N Q11 QB0 R.K ROL RX1 SUPJJ TEORI TR2 UB1 W8V W99 WBKPD WH7 WIH WIK WIN WNSPC WOHZO WQJ WRC WXSBR WYISQ XG1 YFH YUY ZZTAW ~IA ~KM ~WT AAHBH AHBTC AITYG HGLYW OIG 08R IQODW CGR CUY CVF ECM EIF NPM |
ID | FETCH-LOGICAL-f2866-1b7297759e1687837c75741e8aebc669a2ab4ea7d909dc3c27bba5d7059387f73 |
IEDL.DBID | DR2 |
ISSN | 0960-7412 |
IngestDate | Thu May 23 23:13:08 EDT 2024 Sun Oct 22 16:03:46 EDT 2023 Sat Aug 24 00:52:27 EDT 2024 Wed Dec 27 19:15:20 EST 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Keywords | Catalytic subunit Enzyme Arabidopsis epigenetic Transferases Homologous recombination DNA polymerase alpha Arabidopsis thaliana Gene silencing Nucleotidyltransferases Cruciferae Dicotyledones DNA Angiospermae Epigenetics DNA methylation Spermatophyta Mutation Methylation DNA-directed DNA polymerase |
Language | English |
License | CC BY 4.0 |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-f2866-1b7297759e1687837c75741e8aebc669a2ab4ea7d909dc3c27bba5d7059387f73 |
Notes | http://dx.doi.org/10.1111/j.1365-313X.2009.04026.x |
PMID | 19769574 |
PageCount | 10 |
ParticipantIDs | pubmed_primary_19769574 pascalfrancis_primary_22259791 wiley_primary_10_1111_j_1365_313X_2009_04026_x_TPJ4026 fao_agris_US201301724519 |
PublicationCentury | 2000 |
PublicationDate | 2010 January 2010 2010-Jan |
PublicationDateYYYYMMDD | 2010-01-01 |
PublicationDate_xml | – year: 2010 text: 2010 |
PublicationDecade | 2010 |
PublicationPlace | Oxford, UK |
PublicationPlace_xml | – name: Oxford, UK – name: Oxford – name: England |
PublicationTitle | The Plant journal : for cell and molecular biology |
PublicationTitleAlternate | Plant J |
PublicationYear | 2010 |
Publisher | Oxford, UK : Blackwell Publishing Ltd Blackwell Publishing Ltd Blackwell |
Publisher_xml | – name: Oxford, UK : Blackwell Publishing Ltd – name: Blackwell Publishing Ltd – name: Blackwell |
References | 2009; 23 2007; 19 2007; 129 2009; 21 2007; 447 2000; 25 2008; 18 2006; 38 2002; 111 2004; 24 2005; 40 2006; 5 2006; 18 2009; 150 1998; 67 2009; 457 2001; 20 2009; 28 1995; 7 2001; 276 2000; 408 2009; 10 2004; 18 2000; 12 2003; 8 2008; 27 2007; 8 2008; 658 2003; 3 1999; 96 |
References_xml | – volume: 408 start-page: 221 year: 2000 end-page: 225 article-title: PCNA connects DNA replication to epigenetic inheritance in yeast publication-title: Nature – volume: 18 start-page: 782 year: 2004 end-page: 793 article-title: BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis publication-title: Genes Dev. – volume: 24 start-page: 6891 year: 2004 end-page: 6899 article-title: Role of DNA replication proteins in double‐strand break‐induced recombination in Saccharomyces cerevisiae publication-title: Mol. Cell. Biol. – volume: 18 start-page: 521 year: 2008 end-page: 527 article-title: Dividing the workload at a eukaryotic replication fork publication-title: Trends Cell Biol. – volume: 18 start-page: 85 year: 2006 end-page: 103 article-title: ROR1/RPA2A, a putative replication protein A2, functions in epigenetic gene silencing and in regulation of meristem development in Arabidopsis publication-title: Plant Cell – volume: 8 start-page: 77 year: 2007 end-page: 83 article-title: The protein kinase TOUSLED is required for maintenance of transcriptional gene silencing in Arabidopsis publication-title: EMBO Rep. – volume: 20 start-page: 2857 year: 2001 end-page: 2866 article-title: A role for DNA polymerase alpha in epigenetic control of transcriptional silencing in fission yeast publication-title: EMBO J. – volume: 447 start-page: 735 year: 2007 end-page: 738 article-title: Control of DNA methylation and heterochromatic silencing by histone H2B deubiquitination publication-title: Nature – volume: 129 start-page: 665 year: 2007 end-page: 679 article-title: PCNA, the maestro of the replication fork publication-title: Cell – volume: 10 start-page: 192 year: 2009 end-page: 206 article-title: Epigenetic inheritance during the cell cycle publication-title: Nat. Rev. Mol. Cell Biol. – volume: 8 start-page: 394 year: 2003 end-page: 401 article-title: Shoot apical meristem maintenance: the art of a dynamic balance publication-title: Trends Plant Sci. – volume: 23 start-page: 318 year: 2009 end-page: 330 article-title: NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA‐directed DNA methylation publication-title: Genes Dev. – volume: 67 start-page: 721 year: 1998 end-page: 751 article-title: The DNA replication fork in eukaryotic cells publication-title: Annu. Rev. Biochem. – volume: 38 start-page: 706 year: 2006 end-page: 710 article-title: Epigenetic maintenance of the vernalized state in Arabidopsis thaliana requires LIKE HETEROCHROMATIN PROTEIN 1 publication-title: Nat. Genet. – volume: 457 start-page: 413 year: 2009 end-page: 420 article-title: Small RNAs in transcriptional gene silencing and genome defence publication-title: Nature – volume: 7 start-page: 203 year: 1995 end-page: 210 article-title: Induction of homologous DNA recombination in whole plants publication-title: Plant J. – volume: 5 start-page: 1021 year: 2006 end-page: 1029 article-title: Differential usage of non‐homologous end‐joining and homologous recombination in double strand break repair publication-title: DNA Repair (Amst) – volume: 21 start-page: 386 year: 2009 end-page: 402 article-title: Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase {epsilon} Mutation in Arabidopsis publication-title: Plant Cell – volume: 40 start-page: 115 year: 2005 end-page: 128 article-title: DNA polymerases that propagate the eukaryotic DNA replication fork publication-title: Crit. Rev. Biochem. Mol. Biol. – volume: 3 start-page: 21 year: 2003 end-page: 33 article-title: The DNA polymerase alpha‐primase complex: multiple functions and interactions publication-title: ScientificWorldJournal – volume: 276 start-page: 47814 year: 2001 end-page: 47821 article-title: Chromodomain protein Swi6‐mediated role of DNA polymerase alpha in establishment of silencing in fission Yeast publication-title: J. Biol. Chem. – volume: 25 start-page: 143 year: 2000 end-page: 147 article-title: Eukaryotic DNA polymerases, a growing family publication-title: Trends Biochem. Sci. – volume: 19 start-page: 2822 year: 2007 end-page: 2838 article-title: INCURVATA2 encodes the catalytic subunit of DNA Polymerase alpha and interacts with genes involved in chromatin‐mediated cellular memory in Arabidopsis thaliana publication-title: Plant Cell – volume: 150 start-page: 1990 year: 2009 end-page: 1996 article-title: Transcriptional gene silencing mediated by a plastid inner envelope phosphoenolpyruvate/phosphate translocator CUE1 in Arabidopsis publication-title: Plant Physiol. – volume: 96 start-page: 575 year: 1999 end-page: 585 article-title: Replication‐dependent marking of DNA by PCNA facilitates CAF‐1‐coupled inheritance of chromatin publication-title: Cell – volume: 658 start-page: 259 year: 2008 end-page: 270 article-title: What histone code for DNA repair? publication-title: Mutat. Res. – volume: 21 start-page: 367 year: 2009 end-page: 376 article-title: RNA‐mediated chromatin‐based silencing in plants publication-title: Curr. Opin. Cell Biol. – volume: 27 start-page: 1875 year: 2008 end-page: 1885 article-title: Break dosage, cell cycle stage and DNA replication influence DNA double strand break response publication-title: EMBO J. – volume: 111 start-page: 803 year: 2002 end-page: 814 article-title: ROS1, a repressor of transcriptional gene silencing in Arabidopsis, encodes a DNA glycosylase/lyase publication-title: Cell – volume: 12 start-page: 1165 year: 2000 end-page: 1178 article-title: Endogenous targets of transcriptional gene silencing in Arabidopsis publication-title: Plant Cell – volume: 96 start-page: 415 year: 1999 end-page: 424 article-title: Double‐strand break repair in yeast requires both leading and lagging strand DNA polymerases publication-title: Cell – volume: 28 start-page: 1078 year: 2009 end-page: 1086 article-title: An Arabidopsis jmjC domain protein protects transcribed genes from DNA methylation at CHG sites publication-title: EMBO J. |
SSID | ssj0017364 |
Score | 2.2277927 |
Snippet | REPRESSOR OF SILENCING 1 (ROS1) encodes a DNA demethylase that actively removes DNA methylation. Mutation in ROS1 leads to transcriptional gene silencing of a... Summary REPRESSOR OF SILENCING 1 (ROS1) encodes a DNA demethylase that actively removes DNA methylation. Mutation in ROS1 leads to transcriptional gene... |
SourceID | pubmed pascalfrancis wiley fao |
SourceType | Index Database Publisher |
StartPage | 36 |
SubjectTerms | Arabidopsis Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis Proteins - physiology Biological and medical sciences Blotting, Northern Blotting, Southern Blotting, Western Catalytic Domain - genetics Catalytic Domain - physiology Cell Cycle - genetics Cell Cycle - physiology Chromatin Immunoprecipitation DNA Methylation DNA polymerase alpha DNA Polymerase I - genetics DNA Polymerase I - metabolism DNA Polymerase I - physiology DNA-directed DNA polymerase epigenetic epigenetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant - genetics Gene Expression Regulation, Plant - physiology homologous recombination Mutation Nuclear Proteins - genetics Plant physiology and development Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Plants, Genetically Modified - metabolism Promoter Regions, Genetic - genetics Recombination, Genetic - genetics Reverse Transcriptase Polymerase Chain Reaction |
Title | Mutation in the catalytic subunit of DNA polymerase α influences transcriptional gene silencing and homologous recombination in Arabidopsis |
URI | https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1365-313X.2009.04026.x https://www.ncbi.nlm.nih.gov/pubmed/19769574 |
Volume | 61 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3JTuQwELUQ4sBlWIaBZpMPc00rqx0fmwGEkEAIaKlvke04TAtIWrgj0ZyQuPMv8yN8BF9CVRyaRXNC3CLZWZxnV70ql6sI-R0rw0IVh57wE9-LFe7v6kJ5kfa1Abqfah_PDh8ds4N-fDhIBm38E56Fcfkhpg43XBmNvMYFLpX9uMgxQisKokGbdhJMIdZFPol59ZAfnU4zSQU8cpmkgLB7oEQ_BfX890GgbQpZYbCktPC_Clfo4p2mes9mG3W0v0AuXwfiolAuu_VYdfXdpxyP3zPSRfKjZa2056bZEpkx5TKZ26mAWU5-ksej2m3o02FJgVDSxic0gb7U1qoGoUGrgu4e9-ioupqgF8ya5_uHp3_Qvy2RYukYlearCIN3wcQ21A7xTBRoVyrLnP6trlFUV7WlaMdfg1E_fW3vRqphXo3s0K6Q_v7e-Z8Dr63z4BVhypgXKGD4nCfCBCzlYDFrngBGJpVGacaEDKWKjeS58EWuIx1ypWSSc6xGmPKCR7_IbFmVZo3QSCS-STR0ESJmWqWF5hIWiwYWFCqRd8gaYJrJC5CgWf8sxH1boHCYY6dDtj8AnY1cuo8MLWLBRdAhqw75aUsAXE7Ap3YIa_B7a3izrAC5DJHDep8ia5DLbrPzk0O8Wv_qjRtk3oUxoC9ok8yOb2qzBexorLabef8CTAwE1Q |
link.rule.ids | 315,786,790,1382,4043,27956,27957,27958,46329,46753 |
linkProvider | Wiley-Blackwell |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lj9MwEB6hBQkuvGHLY_GBa6o87fhYHquybCsErdSbZTsOVOwm1aaRKCck7vwX_gg_gl_CTJztPsQJcYsU5-F8mZlvxuMZgOepcTw2aRzIMAuD1ND6ri1NkNjQOqT7uQ1p7_Bkysfz9GCRLfp2QLQXxteH2AbcSDI6fU0CTgHpi1JOKVpJlCz6upPoC_EhEsqrKP1Z51-939aSikTia0khZQ_QjF5K6_nrndDelLqmdEnd4BcrfauLc7bqPJ_tDNL-LTg6nYrPQ_k8bNdmaL9eqvL4n-Z6G272xJWN_J92B6646i5ce1Ejudzcgx-T1q_ps2XFkFOyLiy0wbGsaU2LeoPVJXs1HbFVfbShQFjjfn_7_usnju-7pDRsTXbzVIvhs_DfdqxZ0rYoNLBMVwX7VB-Ttq7bhpErf4x-_faxoxNtlkW9apbNfZjvv569HAd9q4egjHPOg8ggyRciky7iuUCn2YoMQXK5dsZyLnWsTeq0KGQoC5vYWBijs0JQQ8JclCJ5ADtVXbldYInMQpdZHCJlyq3JSys0yotFIhQbWQxgF0FV-iMqUTX_ENPSLbI4KrMzgL0LSKuVr_ihyCmWQkYDeOih356JkM5JfNUB8A7AsxNnzhUipwg5avkpVYec-qJm7w7o6NG_XvgMro9nk0N1-Gb69jHc8FkNFBp6Ajvrk9Y9RbK0NnudEPwBrbEI9w |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwEB6hghAX3tDlUXzgmlWednxcWFal0FUFXWlvlu04ZVWarJqNxHJC4s5_4Y_wI_glzMTp9iFOiFskOw_n88x8Y49nAF6mxvHYpHEgwywMUkP7u7Y0QWJD65Du5zaks8P7U747S_fm2byPf6KzMD4_xGbBjSSj09ck4MuivCzkFKGVRMm8TzuJrhAfIp-8nvIkphk-_rBJJRWJxKeSQsYeoBW9EtXz1yehuSl1TdGSusEfVvpKFxdM1UU629mjyR04PhuJD0M5HrYrM7RfryR5_D9DvQu3e9rKRn6e3YNrrroPN17VSC3XD-DHfut39NmiYsgoWbcotMa-rGlNi1qD1SUbT0dsWX9e0zJY435_-_7rJ_bva6Q0bEVW80yH4btwZjvWLOhQFJpXpquCfapPSFfXbcPIkT9Br37z2tGpNouiXjaL5iHMJm8OX-8GfaGHoIxzzoPIIMUXIpMu4rlAl9mKDDFyuXbGci51rE3qtChkKAub2FgYo7NCUDnCXJQieQRbVV25bWCJzEKXWewiZcqtyUsrNEqLRRoUG1kMYBsxVfoIVaiafYxp4xY5HCXZGcDOJaDV0uf7UOQSSyGjATz2yG9aIiRzEj91ALzD77zh3LVC5BQhRwU_peqQU1_U4cEeXT351xtfwM2D8US9fzt99xRu-ZAGWhd6Blur09Y9R6a0MjudCPwBiGwHpg |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Mutation+in+the+catalytic+subunit+of+DNA+polymerase+%CE%B1+influences+transcriptional+gene+silencing+and+homologous+recombination+in+Arabidopsis&rft.jtitle=The+Plant+journal+%3A+for+cell+and+molecular+biology&rft.au=Liu%2C+Jun&rft.au=Ren%2C+Xiaozhi&rft.au=Yin%2C+Haibo&rft.au=Wang%2C+Yu&rft.date=2010&rft.pub=Oxford%2C+UK+%3A+Blackwell+Publishing+Ltd&rft.issn=0960-7412&rft.eissn=1365-313X&rft.volume=61&rft.issue=1&rft.spage=36&rft.epage=45&rft_id=info:doi/10.1111%2Fj.1365-313X.2009.04026.x&rft.externalDocID=US201301724519 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0960-7412&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0960-7412&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0960-7412&client=summon |