Kinetic Scaffolding Mediated by a Phospholipase C-β and Gq Signaling Complex

Transmembrane signals initiated by a broad range of extracellular stimuli converge on nodes that regulate phospholipase C (PLC)-dependent inositol lipid hydrolysis for signal propagation. We describe how heterotrimeric guanine nucleotide-binding proteins (G proteins) activate PLC-βs and in turn are...

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Published inScience (American Association for the Advancement of Science) Vol. 330; no. 6006; pp. 974 - 980
Main Authors Waldo, Gary L, Ricks, Tiffany K, Hicks, Stephanie N, Cheever, Matthew L, Kawano, Takeharu, Tsuboi, Kazuhito, Wang, Xiaoyue, Montell, Craig, Kozasa, Tohru, Sondek, John, Harden, T. Kendall
Format Journal Article
LanguageEnglish
Published Washington, DC American Association for the Advancement of Science 12.11.2010
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Summary:Transmembrane signals initiated by a broad range of extracellular stimuli converge on nodes that regulate phospholipase C (PLC)-dependent inositol lipid hydrolysis for signal propagation. We describe how heterotrimeric guanine nucleotide-binding proteins (G proteins) activate PLC-βs and in turn are deactivated by these downstream effectors. The 2.7-angstrom structure of PLC-β3 bound to activated Gαq reveals a conserved module found within PLC-βs and other effectors optimized for rapid engagement of activated G proteins. The active site of PLC-β3 in the complex is occluded by an intramolecular plug that is likely removed upon G protein-dependent anchoring and orientation of the lipase at membrane surfaces. A second domain of PLC-β3 subsequently accelerates guanosine triphosphate hydrolysis by Gαq, causing the complex to dissociate and terminate signal propagation. Mutations within this domain dramatically delay signal termination in vitro and in vivo. Consequently, this work suggests a dynamic catch-and-release mechanism used to sharpen spatiotemporal signals mediated by diverse sensory inputs.
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ISSN:0036-8075
1095-9203
DOI:10.1126/science.1193438