Expression of Escherichia coli Heat-labile Enterotoxin B Subunit (LTB) in Saccharomyces cerevisiae

• Published in: The journal of microbiology Vol. 43; no. 4; pp. 354 - 360
• Main Authors: Rezaee, Mohammad Ahangarzadeh (Tarbiat Modarres University, Tehran, Iran), Rezaee, Abbas (Tarbiat Modarres University, Tehran, Iran), E-mail: abbasrezaee@yahoo.com, Moazzeni, Seyed Mohammad (Tarbiat Modarres University, Tehran, Iran), Salmanian, Ali Hatef (National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran), Yasuda, Yoko (Nagoya City University Medical School, Nagoya, Japan), Tochikubo, Kunio (Nagoya City University Medical School, Nagoya, Japan), Pirayeh, Shahin Najar (Tarbiat Modarres University, Tehran, Iran), Arzanlou, Mohsen (Tarbiat Modarres University, Tehran, Iran)
• Format: Journal Article
• Language: English
• Published: Korea (South) 01.08.2005
• Subjects: Bacterial Toxins - biosynthesis; Bacterial Toxins - genetics; Bacterial Vaccines - biosynthesis; Bacterial Vaccines - genetics; Cloning, Molecular; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Electrophoresis, Polyacrylamide Gel; enterotoxigenic Escherichia coli; Enterotoxins - biosynthesis; Enterotoxins - genetics; Enzyme-Linked Immunosorbent Assay; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Infections - immunology; Escherichia coli Infections - prevention & control; Escherichia coli Proteins - biosynthesis; Escherichia coli Proteins - genetics; EXPRESION GENICA; EXPRESSION DES GENES; GENE EXPRESSION; Genetic Vectors; Humans; IMMUNOBLOTTING; LTB; Plasmids - genetics; Polymerase Chain Reaction; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Transformation, Genetic; Vaccines, Subunit - immunology
• ISSN: 1225-8873; 1976-3794

Heat-labile enterotoxin B subunit (LTB) of enterotoxigenic Escherichia coli (ETEC) is both a strong mucosal adjuvant and immunogen. It is a subunit vaccine candidate to be used against ETEC-induced diarrhea. It has already been expressed in several bacterial and plant systems. In order to construct yeast expressing vector for the LTB protein, the eltB gene encoding LTB was amplified from a human origin enterotoxigenic E. coli DNA by PCR. The expression plasmid pLTB83 was constructed by inserting the eltB gene into the pYES2 shuttle vector immediately downstream of the GAL1 promoter.