The isolated complex of the translocase of the outer membrane of mitochondria. Characterization of the cation-selective and voltage-gated preprotein-conducting pore

• Published in: The Journal of biological chemistry Vol. 273; no. 47; pp. 31032 - 31039
• Main Authors: Kunkele, K.P. (Universitat Munchen, Munich, FRG.), Juin, P, Pompa, C, Nargana, F.E, Nargang, F.E, Henry, J.P, Neupert, W, Lill, R, Thieffry, M
• Format: Journal Article
• Language: English
• Published: United States 20.11.1998
• Subjects: Biological Transport; Carrier Proteins - isolation & purification; Carrier Proteins - metabolism; Cations - metabolism; CELL MEMBRANES; Cell Polarity; CONDUCTIVIDAD ELECTRICA; CONDUCTIVITE ELECTRIQUE; Electric Conductivity; ELECTRIC CURRENT; ELECTRICAL CONDUCTIVITY; ELECTRICIDAD; ELECTRICITE; ELECTRICITY; ELECTROPHYSIOLOGY; ION; Ion Channel Gating; Ion Channels - isolation & purification; Ion Channels - metabolism; ION TRANSPORT; IONES; IONS; Lipid Bilayers; MEMBRANAS CELULARES; MEMBRANE CELLULAIRE; Membrane Proteins - immunology; Membrane Proteins - metabolism; Membrane Transport Proteins; METABOLISME DES PROTEINES; METABOLISMO PROTEICO; MITOCHONDRIA; Mitochondria - metabolism; Mitochondrial Membrane Transport Proteins; MITOCHONDRIE; MITOCONDRIA; NEUROSPORA CRASSA; Neurospora crassa - metabolism; PEPTIDE; PEPTIDES; PEPTIDOS; POTASIO; POTASSIUM; PRECURSORS; PROTEIN METABOLISM; Protein Precursors - metabolism; Protein Sorting Signals - metabolism; PROTEIN TRANSPORT; PROTEINAS; PROTEINE; PROTEINS; Saccharomyces cerevisiae Proteins; SIGNAL PEPTIDE; Subcellular Fractions - metabolism

The complex of the translocase mitochondrial outer membrane (TOM), mediates recognition, unfolding, and translocation of preproteins. We have used a combination of biochemical and electrophysiological methods to study the properties of the preprotein-conducting pore of the purified TOM complex. The...