Characterization of the Proteolytic Activity of Bacteria Isolated from a Rotating Biological Contactor
Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni26,36,39 and 49 were identified as B. vallismortis, B. subtilis, Aetomonas hydrophila and B.amyloliquefaciens, respectively, based on their biochemical properties and 1...
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Published in | The journal of microbiology Vol. 41; no. 2; pp. 73 - 77 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
한국미생물학회
01.06.2003
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Abstract | Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni26,36,39 and 49 were identified as B. vallismortis, B. subtilis, Aetomonas hydrophila and B.amyloliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses |
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AbstractList | Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni26,36,39 and 49 were identified as B. vallismortis, B. subtilis, Aetomonas hydrophila and B.amyloliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni 26, 36, 39 and 49 were identified as B. vallismortis, B. subtilis, Aeromonas hydrophila and B. amyloliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses. The optimal proteolytic activity was observed in the temperature and pH ranges of 40-70ºC and 8.0-8.5, respectively. The proteolytic activities of all the isolates were partially inhibited by phenylmethylsulfonylfluoride (PMSF), and the isolates Ni 26, Ni 39 and Ni 49 were inhibited by the metalloprotease inhibitor, 1,10-phenanthroline. Zymographic analyses of the culture supernatants revealed the presence of at least two proteases in all isolates. KCI Citation Count: 8 |
Author | Kim, E.H. (Hong-Ik University, Seoul, Republic of Korea) Park, I.J Shin, K.S. (Daejeon University, Daejeon, Republic of Korea) Cho, Y.J. (Seil Technology Co., LTD, Seoul, Republic of Korea) Park, S.J Yoon, J.C |
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Snippet | Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni26,36,39 and 49 were... Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni 26, 36, 39 and 49 were... |
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Title | Characterization of the Proteolytic Activity of Bacteria Isolated from a Rotating Biological Contactor |
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