Characterization of the Proteolytic Activity of Bacteria Isolated from a Rotating Biological Contactor

Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni26,36,39 and 49 were identified as B. vallismortis, B. subtilis, Aetomonas hydrophila and B.amyloliquefaciens, respectively, based on their biochemical properties and 1...

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Published inThe journal of microbiology Vol. 41; no. 2; pp. 73 - 77
Main Authors Park, I.J, Yoon, J.C, Park, S.J, Shin, K.S. (Daejeon University, Daejeon, Republic of Korea), Kim, E.H. (Hong-Ik University, Seoul, Republic of Korea), Cho, Y.J. (Seil Technology Co., LTD, Seoul, Republic of Korea)
Format Journal Article
LanguageEnglish
Published 한국미생물학회 01.06.2003
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Abstract Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni26,36,39 and 49 were identified as B. vallismortis, B. subtilis, Aetomonas hydrophila and B.amyloliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses
AbstractList Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni26,36,39 and 49 were identified as B. vallismortis, B. subtilis, Aetomonas hydrophila and B.amyloliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses
Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni 26, 36, 39 and 49 were identified as B. vallismortis, B. subtilis, Aeromonas hydrophila and B. amyloliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses. The optimal proteolytic activity was observed in the temperature and pH ranges of 40-70ºC and 8.0-8.5, respectively. The proteolytic activities of all the isolates were partially inhibited by phenylmethylsulfonylfluoride (PMSF), and the isolates Ni 26, Ni 39 and Ni 49 were inhibited by the metalloprotease inhibitor, 1,10-phenanthroline. Zymographic analyses of the culture supernatants revealed the presence of at least two proteases in all isolates. KCI Citation Count: 8
Author Kim, E.H. (Hong-Ik University, Seoul, Republic of Korea)
Park, I.J
Shin, K.S. (Daejeon University, Daejeon, Republic of Korea)
Cho, Y.J. (Seil Technology Co., LTD, Seoul, Republic of Korea)
Park, S.J
Yoon, J.C
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Snippet Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni26,36,39 and 49 were...
Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni 26, 36, 39 and 49 were...
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SubjectTerms AEROMONAS
BACILLUS
생물학
Title Characterization of the Proteolytic Activity of Bacteria Isolated from a Rotating Biological Contactor
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