Immunomodulating Activity of the Exopolymer from Submerged Mycelial Culture of Phellinus pini

• Published in: Journal of microbiology and biotechnology Vol. 14; no. 1; pp. 15 - 21
• Main Authors: Jeong, S.C. (Daegu University, Gyungsan, Republic of Korea), Cho, S.P. (Daegu Technical College, Daegu, Republic of Korea), RA, K.S. (Oscotec Inc., Chonan, Republic of Korea), Yang, B.K. (Daegu University, Gyungsan, Republic of Korea), Jeong, Y.T. (Daegu University, Gyungsan, Republic of Korea), Song, C.H. (Daegu University, Gyungsan, Republic of Korea), E-mail: chsong@daegu.ac.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.02.2004
• Subjects: Anticomplementary activity; Biological and medical sciences; Biotechnology; exopolymer; Fundamental and applied biological sciences. Psychology; MACROFAGOS; MACROPHAGE; macrophage activity; MACROPHAGES; Phellinus pini; splenocyte proliferation activity; Fungi; Phellinus; macrophage activity; Phellinus pini; Submerged culture; Immunomodulation; Anticomplementary activity; Basidiomycetes; Polymer; splenocyte proliferation activity; exopolymer; Thallophyta
• ISSN: 1017-7825

The immunomodulating activities and chemical characteristics of a water-soluble exopolymer from submerged mycelial culture of Phellinus pini were studied. Anticomplementary activity of this polymer was found to be 73.2%, and its activation system occurred through both classical and alternative pathways, where the classical pathway was detected to be the major one by crossed immunoelectrophoresis. Nitric oxide (NO) release ability and acid phosphatase activity of macrophage were increased by 1.6-fold (100 μg/ml) and 3.4-fold (500 μg/ml), respectively, and splenocyte proliferation in mixed lymphocyte reaction (MLR) was also increased by 2.6-fold (200 μg/ml), compared to the control. The molecular weight of this polymer, determined by HPLC, was under 5 kDa.