Karyotype Analysis of Three Brassica Species Using Five Different Repetitive DNA Markers by Fluorescence in situ Hybridization

• Published in: Weon'ye gwahag gi'sulji Vol. 27; no. 3; pp. 456 - 463
• Main Authors: Hwang, Y.J., Kyungpook National University, Daegu, Republic of Korea, Kim, H.H., Sahmyook University, Seoul, Republic of Korea, Kwon, S.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Yang, T.J., Seoul National University, Seoul, Republic of Korea, Ko, H.C., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Park, B.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Chung, J.D., Kyungpook National University, Daegu, Republic of Korea, Lim, K.B., Kyungpook National University, Daegu, Republic of Korea
• Format: Journal Article
• Language: English
• Published: 한국원예학회HST 01.09.2009; 한국원예학회
• Subjects: Bleached DAPI Band (BDB); centromere repeats; Cot-100; HETEROCHROMATIN; HETEROCHROMATINE; HETEROCROMATINA; rDNA; 농학; rDNA; centromere repeats; 염색체; Bleached DAPI Band (BDB); BDB 밴드; Cot-100; heterochromatin
• ISSN: 1226-8763; 2465-8588

The karyotypes of three Brassica species, B. rapa (AA, 2n=20), B. nigra (BB, 2n=16), and B. oleracea (CC, 2n=18), were constructed based on fluorescence in situ hybridization (FISH), using various DNA probes, including 5S rDNA, 45S rDNA, 176 bp centromere satellite repeats, and Cot-100 DNA. The lengths of the somatic metaphase chromosomes ranged from 2.05 to 4.21 ㎛ in B. rapa, 2.37 to 3.13 ㎛ in B. nigra, and 2.94 to 4.44 ㎛ in B. oleracea. The karyotypes were composed of eight pairs of metacentrics and two pairs of submetacentrics in B. rapa, five pairs of metacentrics and three pairs of submetacentrics in B. nigra, and six pairs of metacentrics and three pairs of submetacentrics in B. oleracea. Ten 45S rDNA loci were detected in B. rapa, whereas six were detected in B. nigra, and four were detected in B. oleracea. In addition, six 5S rDNA loci were detected in B. rapa, whereas two 5S rDNA loci were detected in both B. nigra and B. oleracea. Both CentBr1 and CentBr2 originated from the B. rapa centromere sequence were detected in nine and five pairs of chromosomes in B. oleracea, respectively; however, they were not observed in B. nigra. Cot-100Br signals were detected on the centromeric regions of all chromosomes in the three Brassica species; however, BDB signals appeared in the centromere region of B. rapa and B. oleracea, but not in B. nigra.