Cytoplasmic free Ca2+ in the marine alga Acetabularia: measurement with Ca2+-selective microelectrodes and kinetic analysis

• Published in: Journal of experimental botany Vol. 43; no. 252; pp. 875 - 885
• Main Authors: Amtmann, A, Klieber, H.G, Gradmann, D
• Format: Journal Article
• Language: English
• Published: 01.07.1992
• Subjects: acetabularia acetabulum; active transport; algorithms; buffers; calcium; chelating agents; Chlorophycota; cytoplasm; efflux; ethylene glycol tetraacetic acid; inorganic ions; ion transport; membrane potential; plasma membrane; specific ion electrodes

Neutral carrier-based Ca2+-selective microelectrodes have been examined for application in concentrated multi-ion solutions. Calculations with data from the literature and our calibration series with Ca2+-EGTA buffers (a convenient algorithm for their calculation is given) provide the physico-chemic...