Comparison of serological tests for detection of infectious bursal disease virus in bursal samples

Six groups of chicks of different breeds/lines viz. Aseel X Dahlem Red (AI), Kadaknath X Dahlem Red (KI), Naked Neck X Dahlem Red (NI), Frizzle X Dahlem Red (FI), commercial layer line of WLH (CLI) and commercial broiler line of WLH (CBI) were experimentally infected with IBDV. Pooled bursal samples...

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Bibliographic Details
Published inIndian veterinary journal Vol. 80; no. 8
Main Authors Kadam, M, Jhala, M.K. (Veterinary College, Anand (India). Department of Veterinary Microbiology)
Format Journal Article
LanguageEnglish
Published 01.08.2003
Subjects
DIAGNOSIS
DIAGNOSTIC
DIAGNÓSTICO
ELISA
ENFERMEDAD DE GUMBORO
GUMBORO DISEASE
IMMUNOELECTROPHORESIS
IMMUNOLOGIE
IMMUNOLOGY
IMMUNOÉLECTROPHORÈSE
INMUNOELECTROFÓRESIS
INMUNOLOGÍA
MALADIE DE GUMBORO
TEST ELISA
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Summary:Six groups of chicks of different breeds/lines viz. Aseel X Dahlem Red (AI), Kadaknath X Dahlem Red (KI), Naked Neck X Dahlem Red (NI), Frizzle X Dahlem Red (FI), commercial layer line of WLH (CLI) and commercial broiler line of WLH (CBI) were experimentally infected with IBDV. Pooled bursal samples were collected from each group at 2nd, 4th, 7th, 10th, 14th day PI. Total 30 samples were tested for the presence of IBDV antigen by AGID, CIEP and Dot ELISA tests. The results of Dot ELISA and CIEP were similar. The sensitivity and specificity of Dot ELISA and CIEP were higher than that of AGID.
Bibliography:2004000622
L73
L50
ISSN:0019-6479