Structure-Function Analysis of the WIP Role in T Cell Receptor-stimulated NFAT Activation: EVIDENCE THAT WIP-WASP DISSOCIATION IS NOT REQUIRED AND THAT THE WIP NH₂ TERMINUS IS INHIBITORY

• Published in: The Journal of biological chemistry Vol. 282; no. 41; pp. 30303 - 30310
• Main Authors: Dong, Xiaoyun, Patino-Lopez, Genaro, Candotti, Fabio, Shaw, Stephen
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 12.10.2007
• Subjects: Actins - chemistry; CD3 Complex - biosynthesis; Cytoskeletal Proteins - metabolism; Humans; Interleukin-2 - metabolism; Intracellular Signaling Peptides and Proteins - metabolism; Jurkat Cells; Kinetics; Models, Biological; NFATC Transcription Factors - chemistry; NFATC Transcription Factors - physiology; Protein Structure, Tertiary; Receptors, Antigen, T-Cell - metabolism; Structure-Activity Relationship; Tetradecanoylphorbol Acetate - chemistry; Transcription, Genetic; Wiskott-Aldrich Syndrome - metabolism; Wiskott-Aldrich Syndrome Protein - metabolism

WASP and its binding partner WIP play important roles in T cells both in actin polymerization and in interleukin-2 transcription. Aberrations thereof contribute to the pathology of Wiskott-Aldrich syndrome (WAS). To directly evaluate the cooperativity of WIP and WASP in interleukin-2 transcription,...