Correlation between pyridoxal-5'-phosphate levels and the percentage activation of aspartate aminotransferase enzyme in haemolysate and plasma during in vitro incubation studies with different B6 vitamers

• Published in: Enzyme (Basel) Vol. 35; no. 4; pp. 215 - 224
• Main Authors: Vermaak, W.J.H, Barnard, H.C, Van Dalen, E.M.S.P, Potgieter, G.M
• Format: Journal Article
• Language: English
• Published: Switzerland 1986
• Subjects: Aspartate Aminotransferases - blood; aspartate transaminase; Chromatography, High Pressure Liquid; enzyme activity; erythrocytes; Erythrocytes - metabolism; Hemolysis; Humans; In Vitro Techniques; Plasma - metabolism; Pyridoxal - blood; pyridoxal phosphate; Pyridoxal Phosphate - blood; Pyridoxamine - blood; pyridoxine; Pyridoxine - blood; vitamin deficiencies

Conflicting results using erythrocyte aminotransferase (eAST) stimulation to assess vitamin B6 nutritional status in patients with less severe B6 deficiencies are common. It has been claimed that the presence of different B6 vitamers may modify the activation of eAST by pyridoxal-5'-phosphate (...