yeast aminoacyl tRNA synthetases. methodology for their complete or partial purification and comparison of their relative activities under various extraction conditions

• Published in: Biochimie Vol. 59; no. 5/6; pp. 453 - 462
• Main Authors: Kern, D, Dietrich, A, Fasiolo, F, Renaud, M, Giege, R, Ebel, J.P
• Format: Journal Article
• Language: English
• Published: France 1977
• Subjects: Amino Acyl-tRNA Synthetases - isolation & purification; Phenylalanine-tRNA Ligase - isolation & purification; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Valine-tRNA Ligase - isolation & purification

Several fractionation steps are described which can be applied to the partial purification of the 20 aminoacyl-tRNA synthetases from commercial baker's yeast. Comparative experiments performed in the presence or absence of protease inhibitors revealed that some enzymes prepared in the presence...