Fructan synthesis in excised barley leaves. Identification of two sucrose-sucrose fructosyltransferases induced by light and their separation from constitutive invertases

• Published in: Plant physiology (Bethesda) Vol. 101; no. 2; pp. 459 - 468
• Main Authors: Simmen, U, Obenland, D, Boller, T, Wiemken, A
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.02.1993
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Agronomy. Soil science and plant productions; Anions; Barley; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; Biosynthesis; Chromatography; Enzymes; FEUILLE; Fructans; FRUCTOFURANOSIDASA; FRUCTOFURANOSIDASE; FRUCTOSA; FRUCTOSE; Fundamental and applied biological sciences. Psychology; HOJAS; HORDEUM VULGARE; Leaves; LUMIERE; LUZ; Metabolism; Metabolism and Enzymology; Oligosaccharides; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; Plants; POLISACARIDOS; POLYHOLOSIDE; PURIFICACION; PURIFICATION; SACCHAROSE; SUCROSA; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; TRANSFERASAS; TRANSFERASE; Trisaccharides; Monocotyledones; Hordeum vulgare; Enzyme; β-D-Fructofuranosidase; Biosynthesis; Plant leaf; Cereal crop; Sucrose 1F-fructosyltransferase; Enzymatic activity; Gramineae; Light; Angiospermae; Fructosan; Spermatophyta; Carbohydrate; Biological accumulation

Excised leaves of barley (Hordeum vulgare L.) exposed to continuous light accumulate large amounts of soluble carbohydrates. Carbohydrates were analyzed in deionized extracts by high-pressure liquid chromatography on an anion exchange column coupled with pulsed amperometric detection. During the fir...