Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrowderived mesenchymal stem cells and adiposederived mesenchymal stem cells

• Published in: Journal of animal reproduction & biotechnology (Online) Vol. 39; no. 4; pp. 278 - 293
• Main Authors: Yeon-Ji Lee, In-Won Lee, Tae-Suk Kim, Bo-Gyeong Seo, Sang-Ki Baek, Cheol Hwangbo, Joon-Hee Lee
• Format: Journal Article
• Language: English
• Published: 한국동물생명공학회(구 한국수정란이식학회) 31.12.2024
• Subjects: in vitro differentiation; endothelial cells; bone marrow-derived mesenchymal stem cells; epiblast stem cells; adipose-derived mesenchymal stem cells
• ISSN: 2671-4639; 2671-4663

Background: Pluripotent stem cells (PSCs) are capable of differencing into various cell types in the body, providing them valuable for therapy of degenerative diseases. Patientspecific treatments using PSCs, such as mesenchymal stem cells in patient’s own body, may reduce the risk of immune rejectio...

Table of Contents:

• ABSTRACT INTRODUCTION MATERIALS AND METHODS Stem cells culture Isolation and culture of swine umbilical veinendothelial cells (SUVECs) In vitro differentiation of stem cells into endothelialcells (ECs) Magnetic-activated cells sorting (MACS) Immunocytochemistry Quantitative real-time polymerase chain reaction PCR(qRT-PCR) Flow cytometry (FACS) Capillary-like structure formation assay Three-dimensional spheroid sprouting assay Statistical analysis RESULTS Culture of pEpiSCs, pBM-MSCs and pA-MSCs fordifferentiation In vitro differentiation of pEpiSCs, pBM-MSCs andpA-MSCs into ECs Morphology of cells differentiated from pEpiSCs,pBM-MSCs and pA-MSCs Characterization of ECs differentiated from pEpiSCs,pBM-MSCs and pA-MSCs Expression of pluripotency markers in ECsdifferentiated from pEpiSCs, pBM-MSCs and pAMSCs Capillary-like structure formation of ECs differentiatedfrom pEpiSCs, pBM-MSCs and pA-MSCs Three-dimensional spheroids sprouting of ECsdifferentiated from pEpiSCs, pBM-MSCs and pAMSCs DISCUSSION CONCLUSION REFERENCES