Waking up Streptomyces secondary metabolism by constitutive expression of activators or genetic disruption of repressors

• Published in: Methods in enzymology Vol. 517; p. 343
• Main Authors: Aigle, Bertrand, Corre, Christophe
• Format: Journal Article
• Language: English
• Published: United States 2012
• Subjects: Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological Products - metabolism; Biosynthetic Pathways - genetics; Escherichia coli - genetics; Escherichia coli - metabolism; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Regulator; Genetic Engineering - methods; Macrolides - metabolism; Metabolome; Multigene Family; Peptides - genetics; Peptides - metabolism; Repressor Proteins - genetics; Repressor Proteins - metabolism; Streptomyces - genetics; Streptomyces - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Transcriptional Activation

Streptomycete bacteria are renowned as a prolific source of natural products with diverse biological activities. Production of these metabolites is often subject to transcriptional regulation: the biosynthetic genes remain silent until the required environmental and/or physiological signals occur. C...