Imaging Blood Vessels in the Zebrafish

Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous...

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Bibliographic Details
Published inMethods in Cell Biology Vol. 100; pp. 27 - 54
Main Authors Kamei, Makoto, Isogai, Sumio, Pan, Weijun, Weinstein, Brant M.
Format Book Chapter Journal Article
LanguageEnglish
Published United States Elsevier Science & Technology 2010
Subjects
Angiography
Animals
Animals, Genetically Modified
Blood Vessels - cytology
Danio rerio
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - metabolism
Freshwater
In Situ Hybridization
Time-Lapse Imaging
Zebrafish
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Summary:Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous and deep localization of vessels within embryo development and also in adult tissues. In this chapter, we have summarized various methods for vessel imaging in zebrafish, including in situ hybridization for vascular-specific genes, resin injection- or dye injection-based vessel visualization, and alkaline phosphatase staining. We also described detail protocols for live imaging of vessels by microangiography or using various transgenic zebrafish lines.
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ISBN:012384892X
9780123848925
ISSN:0091-679X
DOI:10.1016/B978-0-12-384892-5.00002-5