A novel 3D heterotypic spheroid model for studying extracellular vesicle-mediated tumour and immune cell communication

• Published in: Biochemical and biophysical research communications Vol. 495; no. 2; pp. 1930 - 1935
• Main Authors: Sadovska, Lilite, Zandberga, Elīna, Sagini, Krizia, Jēkabsons, Kaspars, Riekstiņa, Una, Kalniņa, Zane, Llorente, Alicia, Linē, Aija
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 08.01.2018
• Subjects: 3D heterotypic spheroids; 60 APPLIED LIFE SCIENCES; CELL CULTURES; CULTURE MEDIA; Extracellular vesicles; Immune cells; LYMPHOCYTES; Macropinocytosis; NEOPLASMS; PROSTATE; Prostate cancer; Uptake; EVs; Extracellular vesicles; 3D heterotypic spheroids; FACS; MVs; PBMCs; TME; Uptake; GFP; Immune cells; HD; Prostate cancer; Macropinocytosis

Cancer-derived extracellular vesicles (EVs) have emerged as important mediators of tumour-host interactions, and they have been shown to exert various functional effects in immune cells. In most of the studies on human immune cells, EVs have been isolated from cancer cell culture medium or patients&...