Concentrations of quinolinic acid in cerebrospinal fluid measured by gas chromatography and electron-impact ionisation mass spectrometry: Age-related changes in a paediatric reference population

• Published in: Journal of chromatography. B, Biomedical sciences and applications Vol. 696; no. 1; pp. 53 - 58
• Main Authors: Dobbie, Michael S, Surtees, Robert A.H
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.08.1997; Elsevier Science
• Subjects: Adolescent; Age Factors; Biological and medical sciences; Cerebrospinal fluid (biochemistry, cytology, circulation, manometry). Intracranial pressure determination; Child; Child, Preschool; Gas Chromatography-Mass Spectrometry - methods; Humans; Infant; Infant, Newborn; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Quinolinic acid; Quinolinic Acid - cerebrospinal fluid; Quinolinic Acid - chemistry; Reference Standards; Reference Values; Reproducibility of Results; Sensitivity and Specificity; Quinolinic acid; Human; Biological fluid; Pediatrics; Nervous system diseases; Biological marker; Cerebrospinal fluid; Medical screening; Gas chromatography; Laboratory investigations; Clinical biology; Child; Mass spectrometry; Quantitative analysis

A simple method for the determination of the excitotoxin, quinolinic acid (QUIN) in cerebrospinal fluid (CSF) is described. QUIN, in lyophilized samples, was silylated by N-methyl- N-( tert.-butyldimethylsilyl)trifluoroacetamide in a single-step reaction at 65 to 70°C to form a di- tert.-butyldimeth...