Derivation of a structural model for the c-myc IRES

• Published in: Journal of molecular biology Vol. 310; no. 1; pp. 111 - 126
• Main Authors: Le Quesne, John P.C, Stoneley, Mark, Fraser, Graham A, Willis, Anne E
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 29.06.2001
• Subjects: Base Sequence; Binding Sites; c-myc; c-myc gene; Genes, myc - genetics; HeLa Cells; Humans; internal ribosome entry segment; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Regulatory Sequences, Nucleic Acid - genetics; Ribosomes - metabolism; RNA Stability; RNA structure; RNA, Messenger - chemistry; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Alignment; Sequence Deletion - genetics; Software; Thermodynamics; Transfection; translation; UTR; c-myc; CMCT; IRES; eIF4F; FCS; translation; AMV; DMS; RNA structure; internal ribosome entry segment; CSFV

We have derived a secondary structure model for the c-myc internal ribosome entry segment (IRES) by using information from chemical probing of the c-myc IRES RNA to constrain structure prediction programs. Our data suggest that the IRES is modular in nature, and can be divided into two structural do...