Cloning, expression and biochemical characterisation of a unique thermostable pullulan-hydrolysing enzyme from the hyperthermophilic archaeon Thermococcus aggregans

• Published in: FEMS microbiology letters Vol. 190; no. 2; pp. 223 - 229
• Main Authors: Niehaus, Frank, Peters, Anke, Groudieva, Tatiana, Antranikian, Garabed
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.09.2000
• Subjects: amylopectin; amylose; Archaeon; Escherichia coli; Pullulan hydrolase; Starch; Thermococcales; Thermococcus aggregans; Archaeon; Thermococcales; Pullulan hydrolase; Starch

The gene for a new type of pullulan hydrolase from the hyperthermophilic archaeon Thermococcus aggregans was cloned and expressed in Escherichia coli. The 2181-bp open reading frame encodes a protein of 727 amino acids. A hypothetical membrane linker region was found to be cleaved during processing...