Preparation and metatranscriptomic analyses of host-microbe systems

• Published in: Methods in enzymology Vol. 531; p. 169
• Main Authors: Hampton-Marcell, Jarrad T, Moormann, Stephanie M, Owens, Sarah M, Gilbert, Jack A
• Format: Journal Article
• Language: English
• Published: United States 2013
• Subjects: Genome, Bacterial; Host-Pathogen Interactions; Metabolomics; Metagenomics - methods; Microbial Consortia - genetics; Sequence Analysis, RNA - methods; Systems Biology; Transcriptome; Metatranscriptomics; Translation; Microbial; Host–microbe; Next-generation sequencing

Metatranscriptomics has increased our working knowledge of the functional significance and genetic variability of microbial communities, yet there is still limited information concerning how gene expression and regulation in a microbiome influences interactions with a host organism. During a pathoge...