Integration of bioinformatic and chemoproteomic tools for the study of enzyme conservation in closely related bacterial species

• Published in: Methods in enzymology Vol. 664; p. 1
• Main Authors: Keller, Laura J, Lakemeyer, Markus, Bogyo, Matthew
• Format: Journal Article
• Language: English
• Published: United States 01.01.2022
• Subjects: Animals; Bacteria - genetics; Computational Biology; Hydrolases; Mammals; Microbiota; Proteomics - methods; Enzyme conservation; Activity-based protein profiling; Serine hydrolases; Bioinformatics; Commensal bacteria

Activity-based protein profiling (ABPP) is a commonly utilized technique to globally characterize the endogenous activity of multiple enzymes within a related family. While it has been used extensively to identify enzymes that are differentially active across various mammalian tissues, recent effort...