Generation of polyclonal antiserum against the growth hormone secretagogue receptor (GHS-R): Evidence that the GHS-R exists in the hypothalamus, pituitary and stomach of rats

• Published in: Life sciences (1973) Vol. 68; no. 9; pp. 991 - 996
• Main Authors: Shuto, Yujin, Shibasaki, Tamotsu, Wada, Ken, Parhar, Ishwar, Kamegai, Jun, Sugihara, Hitoshi, Oikawa, Shinichi, Wakabayashi, Ichiji
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 19.01.2001
• Subjects: Animals; Antibody; Antibody Specificity; Blotting, Western; COS Cells - metabolism; Enzyme-Linked Immunosorbent Assay; Gastric Mucosa - metabolism; Ghrelin; Growth hormone secretagogue receptor; Hypothalamus - metabolism; Immune Sera - biosynthesis; Immune Sera - immunology; Immune Sera - isolation & purification; Peptide Fragments - immunology; Pituitary Gland - metabolism; Protein Processing, Post-Translational; Rabbits; Rat; Rats; Receptors, Cell Surface - genetics; Receptors, Cell Surface - immunology; Receptors, Cell Surface - metabolism; Receptors, G-Protein-Coupled; Receptors, Ghrelin; Recombinant Proteins - immunology; Stomach - chemistry; Tissue Distribution; Transfection; Growth hormone secretagogue receptor; Rat; Ghrelin; Antibody

Growth hormone (GH) secretagogues (GHSs), which stimulate GH secretion, are synthetic compounds that act through the GHS receptor (GHS-R) which has been recently cloned. We raised an antiserum in a rabbit against a synthetic peptide corresponding to amino acid residues 248–260 of the third intracell...