Membrane-associated reactions in ubiquinone biosynthesis: 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone methyltransferase

• Published in: Biochimica et biophysica acta Vol. 428; no. 1; pp. 146 - 156
• Main Authors: Leppik, Ray A., Stroobant, Paul, Shineberg, Barry, Young, Ian G., Gibson, Frank
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 25.03.1976
• Subjects: Animals; Cations, Divalent; Cell Membrane - enzymology; Cell Membrane - metabolism; Centrifugation, Density Gradient; Chromatography, Gel; Dithiothreitol - metabolism; Escherichia coli - enzymology; Genes; Methyltransferases - isolation & purification; Methyltransferases - metabolism; Molecular Weight; Mutation; Quinones; Transduction, Genetic; Ubiquinone - biosynthesis; OMHMB

The O-methylation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone, which has been previously postulated to be the final reaction in the biosynthesis of ubiquinone was demonstrated in vitro using cell extracts of Escherichia coli. S- Adenosyl- l- methionine was active as the methyl dono...