Development of a modified subtraction hybridization technique and its application for the design of strain specific PCR systems for lactococci

• Published in: FEMS microbiology letters Vol. 166; no. 1; pp. 63 - 70
• Main Authors: Wassill, Lars, Ludwig, Wolfgang, Schleifer, Karl Heinz
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.1998
• Subjects: Lactococcus lactis; Magnetic separation; Strain specific PCR; Subtraction hybridization; Lactococcus lactis; Magnetic separation; Strain specific PCR; Subtraction hybridization

A modified genomic DNA subtraction hybridization approach for the design of strain specific PCR systems was developed and evaluated. The method is based on the magnetic separation of homologous and heterologous hybrids of amplified reference (subtracter) and target (probe) DNA fragments from probe s...