Yeast lipid analysis and quantification by mass spectrometry

• Published in: Methods in enzymology Vol. 470; p. 369
• Main Authors: Guan, Xue Li, Riezman, Isabelle, Wenk, Markus R, Riezman, Howard
• Format: Journal Article
• Language: English
• Published: United States 2010
• Subjects: Gas Chromatography-Mass Spectrometry; Glycerophospholipids - analysis; Glycerophospholipids - chemistry; Lipids - analysis; Mass Spectrometry - methods; Solid Phase Extraction; Spectrometry, Mass, Electrospray Ionization; Sphingolipids - analysis; Sphingolipids - chemistry; Tandem Mass Spectrometry; Yeasts - chemistry

The systematic and quantitative analysis of the different lipid species within a cell or an organism has recently become possible and the general approach has been termed "lipidomics." Traditional methods of identification and quantification of lipid species were laborious processes and it...