Unauthorised antibiotic treatments in beekeeping: Development and validation of a method to quantify and confirm tylosin residues in honey using liquid chromatography–tandem mass spectrometric detection

• Published in: Analytica chimica acta Vol. 520; no. 1; pp. 87 - 92
• Main Authors: Benetti, C., Dainese, N., Biancotto, G., Piro, R., Mutinelli, F.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 23.08.2004; Elsevier
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Honey; Mass spectrometry; Other chromatographic methods; Spectrometric and optical methods; Tylosin; Validation; Validation; Honey; Tylosin; Mass spectrometry; Ammonium; Internal standard; HPLC chromatography; Acetate; Antibiotic; Sample preparation; Residue; Mass spectrometry MS/MS; Acetonitrile; Solid phase; Quantitative analysis

A liquid chromatographic–tandem mass spectrometric (HPLC-MS/MS) method is proposed for the identification and quantification of tylosin in honey. Sample treatment involves an extraction in a Tris buffer at pH 10.5, followed by a solid-phase clean up step on an Oasis HLB column. Roxithromycin was use...