Liquid chromatography–positive ion electrospray mass spectrometry method for the quantification of citalopram in human plasma

A rapid, sensitive and novel narrow-bore liquid chromatography–mass spectrometric method was developed and fully validated for the quantification of citalopram in human plasma. The analyte and internal standard (imipramine) were extracted by liquid–liquid extraction with a mixture of hexane–heptane–...

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Published inJournal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 810; no. 2; pp. 235 - 244
Main Authors Pistos, Constantinos, Panderi, Irene, Atta-Politou, Julia
Format Journal Article
LanguageEnglish
Japanese
Published Elsevier B.V 25.10.2004
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Abstract A rapid, sensitive and novel narrow-bore liquid chromatography–mass spectrometric method was developed and fully validated for the quantification of citalopram in human plasma. The analyte and internal standard (imipramine) were extracted by liquid–liquid extraction with a mixture of hexane–heptane–isopropanol (88:10:2, v/v/v). The use of a Hypersil BDS C 8 micro-bore column (250 mm × 2.1 mm i.d.; 3.5 μm particle size), results in substantial reduction in solvent consumption. The mobile phase consisted of 10 mM ammonium formate–formic acid (pH 4.5) and acetonitrile (30:70, v/v), pumped at a flow rate of 0.15 ml min −1. The analytes were detected after positive electrospray ionization using the selected ion-monitoring mode of the species at m/ z 325 for citalopram and m/ z 281 for imipramine. The method had a chromatographic run time of 10.0 min and a linear calibration curve over the range 0.50–250 ng ml −1 ( r 2 > 0.996). The limit of quantitation was 0.50 ng ml −1. Accuracy and precision were below the acceptance limits of 15%.
AbstractList A rapid, sensitive and novel narrow-bore liquid chromatography–mass spectrometric method was developed and fully validated for the quantification of citalopram in human plasma. The analyte and internal standard (imipramine) were extracted by liquid–liquid extraction with a mixture of hexane–heptane–isopropanol (88:10:2, v/v/v). The use of a Hypersil BDS C 8 micro-bore column (250 mm × 2.1 mm i.d.; 3.5 μm particle size), results in substantial reduction in solvent consumption. The mobile phase consisted of 10 mM ammonium formate–formic acid (pH 4.5) and acetonitrile (30:70, v/v), pumped at a flow rate of 0.15 ml min −1. The analytes were detected after positive electrospray ionization using the selected ion-monitoring mode of the species at m/ z 325 for citalopram and m/ z 281 for imipramine. The method had a chromatographic run time of 10.0 min and a linear calibration curve over the range 0.50–250 ng ml −1 ( r 2 > 0.996). The limit of quantitation was 0.50 ng ml −1. Accuracy and precision were below the acceptance limits of 15%.
Author Pistos, Constantinos
Atta-Politou, Julia
Panderi, Irene
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Keywords Imipramine
High-performance liquid chromatography
Mass spectrometry
Electrospray ionization
Citalopram
Language English
Japanese
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Snippet A rapid, sensitive and novel narrow-bore liquid chromatography–mass spectrometric method was developed and fully validated for the quantification of citalopram...
SourceID elsevier
SourceType Publisher
StartPage 235
SubjectTerms Citalopram
Electrospray ionization
High-performance liquid chromatography
Imipramine
Mass spectrometry
Title Liquid chromatography–positive ion electrospray mass spectrometry method for the quantification of citalopram in human plasma
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