Studies on the acid-soluble nucleotide pool in thymine-requiring mutants of Escherichia coli during thymine starvation : I. Accumulation of deoxyadenosine triphosphate in Escherichia coli 15 T −A −U

• Published in: Biochimica et biophysica acta Vol. 80; no. 4; pp. 542 - 551
• Main Authors: Munch-Petersen, Agnete, Neuhard, Jan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 27.04.1964
• Subjects: Adenine Nucleotides; Adenosine Triphosphate; Arginine; Chromatography; Colorimetry; Deoxyadenine Nucleotides; Deoxyadenosines; Escherichia coli; Guanine Nucleotides; Metabolism; Mutation; Nucleotides; OldMedline; Perchlorates; Polyphosphates; Ribose; Thymine; Uracil; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; RIBOSE; GUANINE NUCLEOTIDES; THYMINE; CHROMATOGRAPHY; URACIL; ADENINE NUCLEOTIDES; METABOLISM; MUTATION; PERCHLORIC ACIDS; ARGININE; COLORIMETRY; ADENOSINE TRIPHOSPHATE
• DOI: 10.1016/0926-6550(64)90298-1
• ISSN: 0926-6550; 0006-3002

1. 1. The variations in nucleotide content of Escherichia coli 15 T −A −U − (requiring thymine, arginine and uracil) during thymine starvation have been investigated. 90 min after removal of thymine from the medium a 2-fold increase in nucleotide content per cell mass was observed in HClO 4 extracts of culture. 2. 2. The time course of this increase, measured as absorbancy at 260 mμ, was investigated and was shown to be paralleled by a corresponding increase in purine-bound ribose, while purine-bound deoxyribose increased by the same factor, but at a much faster rate. 3. 3. Fractionation of the nucleotides on a column of Dowex-1 (Cl − form) showed that the increase in purine-bound deoxyribose was mainly due to an accumulation of deoxyadenosinetriphosphate and deoxyadenosinediphosphate. No similar increase in deoxyguanosine nucleotides was observed.