New broad-host-range promoter probe vectors based on the plasmid RK2 replicon

• Published in: FEMS microbiology letters Vol. 195; no. 1; pp. 91 - 96
• Main Authors: Santos, Pedro Miguel, Di Bartolo, Ilaria, Blatny, Janet Martha, Zennaro, Elisabetta, Valla, Svein
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05.02.2001
• Subjects: Broad-host-range; Plasmid; Promoter probe; RK2; Vector; Plasmid; RK2; Broad-host-range; Promoter probe; Vector

Broad-host-range plasmid RK2-based promoter probe vectors with a known nucleotide sequence were constructed. In the absence of an upstream promoter, the expression of two tested reporter genes ( luc and lacZ) in Escherichia coli was virtually zero, while insertion of the Ptrc promoter resulted in st...