Evaluation of PCR mediated DNA amplification in non-invasive biological specimens for subclinical detection of Mycobacterium leprae

• Published in: FEMS immunology and medical microbiology Vol. 11; no. 2; pp. 113 - 120
• Main Authors: Santos, Adalberto R., Filho, Jose T.Goes, Nery, Jose A.C., Duppre, Nadia C., Gallo, Maria E.N., Suffys, Philip N., Degrave, Wim M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 1995
• Subjects: Clinical sample; Leprosy diagnosis; Mycobacterium leprae; Polymerase chain reaction; Polymerase chain reaction; Leprosy diagnosis; Mycobacterium leprae; Clinical sample

DNA from Mycobacterium leprae, present in non-invasive clinical samples from leprosy patients, such as nasal secretion and hair bulbs, was submitted to amplification by the polymerase chain reaction using a M. leprae-specific repetitive sequence as a target. After optimization of sample processing a...