Evaluation of cross-protection between O 1 Manisa and O 1 Campos in cattle vaccinated with foot-and-mouth disease virus vaccine incorporating different payloads of inactivated O 1 Manisa antigen
Serology is used to predict vaccine induced protection against challenge with a heterologous strain of the same serotype of foot-and-mouth disease virus (FMDV). To evaluate the accuracy of such predictions, we compared the protection afforded to cattle vaccinated with the O 1 Manisa strain of FMDV a...
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Published in | Vaccine Vol. 29; no. 10; pp. 1906 - 1912 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
24.02.2011
|
Subjects | |
Online Access | Get full text |
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Summary: | Serology is used to predict vaccine induced protection against challenge with a heterologous strain of the same serotype of foot-and-mouth disease virus (FMDV). To evaluate the accuracy of such predictions, we compared the protection afforded to cattle vaccinated with the O
1 Manisa strain of FMDV against challenge with either a homologous (O
1 Manisa) or a heterologous strain (O
1 Campos). Serology by virus neutralization test (VNT) using O
1 Manisa antiserum predicted an acceptable protection against such a challenge. Two experiments were carried out to compare the results for consistency. A total of 78 naïve cattle were vaccinated with different antigen payloads (60–0.94
μg) of O
1 Manisa. They were challenged by intradermolingual inoculation with live FMDV, either O
1 Manisa or O
1 Campos. Unvaccinated naïve control cattle (
n
=
20) were also challenged with either the O
1 Manisa or O
1 Campos viruses and all developed generalized FMD. The protection results for the vaccinated cattle revealed that higher payloads of O
1 Manisa vaccine were needed to protect against heterologous challenge compared to that for homologous challenge. The 50% protective dose (PD
50) values for the vaccine in experiments 1 and 2 were found to be 28.78 and 9.44 for the homologous challenge and 3.98 and 5.01 for heterologous challenge. Furthermore, protection against O
1 Campos required a higher level of vaccine-induced antibody against this virus compared to the level of O
1 Manisa neutralizing antibody associated with protection against homologous challenge. The 50% protective level of
in vitro neutralizing antibody was found to be log
10
1.827 for O
1 Campos and log
10
0.954 for O
1 Manisa based on O
1 Manisa based virus neutralization test. |
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ISSN: | 0264-410X 1873-2518 |
DOI: | 10.1016/j.vaccine.2010.12.127 |