Cytosolic Ca 2+ during atrial natriuretic peptide secretion from cultured neonatal cardiomyocytes

• Published in: Molecular and cellular endocrinology Vol. 73; no. 2; pp. 153 - 163
• Main Authors: Uusimaa, Paavo A., Ruskoaho, Heikki, Leppäluoto, Juhani, Hassinen, Ilmo E.
• Format: Journal Article
• Language: English
• Published: Elsevier Ireland Ltd 22.10.1990
• Subjects: Atrial natriuretic peptide; Calcium; Cell culture; Heart myocytes, rat; Microcarrier; Protein kinase C; Cell culture; Protein kinase C; Atrial natriuretic peptide; Calcium; Microcarrier; Heart myocytes, rat

Mechanisms of atrial natriuretic peptide (ANP) release were studied in neonatal rat heart atrial and ventricular myocytes cultured on Cytodex 3 microcarriers. For simultaneous observations of cytosolic free calcium concentration ([Ca 2+] f) and ANP secretion, the culture was packed in a chromatograp...