FRET-based sensors for the human M 1-, M 3-, and M 5-acetylcholine receptors

• Published in: Bioorganic & medicinal chemistry Vol. 19; no. 3; pp. 1048 - 1054
• Main Authors: Ziegler, Nicole, Bätz, Julia, Zabel, Ulrike, Lohse, Martin J., Hoffmann, Carsten
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.02.2011
• Subjects: FlAsH-EDT 2; FRET; GPCR; Muscarine acetylcholine receptor; Receptor activation; OxoM; FlAsH-EDT 2; mACh; CFP; Carb; DMEM; Receptor activation; FCS; GPCR; Muscarine acetylcholine receptor; FRET; HBSS; FlAsH

Based on the recently developed approach to generate fluorescence resonance energy transfer (FRET)-based sensors to measure GPCR activation, we generated sensor constructs for the human M 1-, M 3-, and M 5-acetylcholine receptor. The receptors were labeled with cyan fluorescent protein (CFP) at thei...