Design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples

• Published in: FEMS microbiology letters Vol. 213; no. 2; pp. 175 - 182
• Main Authors: Wang, Rong-Fu, Beggs, Marjorie L, Robertson, Latriana H, Cerniglia, Carl E
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 06.08.2002
• Subjects: 16S rDNAs amplification; Human intestinal bacterium; Oligonucleotide-microarray; Polymerase chain reaction; Polymerase chain reaction; 16S rDNAs amplification; Human intestinal bacterium; Oligonucleotide-microarray

An oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. The 16S rDNA sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. Three 40-mer oligonucleotides specific f...