A new member of the ribbon-helix-helix transcription factor superfamily from the plant pathogen Xanthomonas axonopodis pv.citri

XACb0070 is an uncharacterized protein coded by the two large plasmids isolated from Xanthomonas axonopodis pv. citri, the agent of citrus canker and responsible for important economical losses in citrus world production. XACb0070 presents sequence homology only with other hypothetical proteins belo...

Full description

Saved in:
Bibliographic Details
Published inJournal of structural biology Vol. 170; no. 1; pp. 21 - 31
Main Authors Gallo, Mariana, Ferrari, Elena, Eliseo, Tommaso, Amata, Irene, Pertinhez, Thelma A., Katsuyama, Angela M., Paci, Maurizio, Farah, Chuck S., Spisni, Alberto, Cicero, Daniel O.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.04.2010
Subjects
Amino Acid Sequence
Antitoxin
Base Sequence
Citrus - microbiology
Citrus canker
Dimerization
DNA-binding protein
Electrophoresis, Polyacrylamide Gel
Models, Molecular
Molecular Sequence Data
NMR
Nuclear Magnetic Resonance, Biomolecular
Plasmids - genetics
Protein Conformation
Ribbon-helix-helix fold
Sequence Analysis, DNA
Spectrometry, Fluorescence
Transcription Factors - genetics
Xanthomonas axonopodis - genetics
τci
Ribbon-helix-helix fold
Citrus canker
J
TA
NMR
DNA-binding protein
RHH
Rh
Antitoxin
CSI
Xaa
rmsd
Xac
Online AccessGet full text

Cover

Abstract XACb0070 is an uncharacterized protein coded by the two large plasmids isolated from Xanthomonas axonopodis pv. citri, the agent of citrus canker and responsible for important economical losses in citrus world production. XACb0070 presents sequence homology only with other hypothetical proteins belonging to plant pathogens, none of which have their structure determined. The NMR-derived solution structure reveals this protein is a homodimer in which each monomer presents two domains with different structural and dynamic properties: a folded N-terminal domain with βαα topology which mediates dimerization and a long disordered C-terminal tail. The folded domain shows high structural similarity to the ribbon-helix-helix transcriptional repressors, a family of DNA-binding proteins of conserved 3D fold but low sequence homology: indeed XACb0070 binds DNA. Primary sequence and fold comparison of XACb0070 with other proteins of the ribbon-helix-helix family together with examination of the genes in the vicinity of xacb0070 suggest the protein might be the component of a toxin–antitoxin system.
AbstractList XACb0070 is an uncharacterized protein coded by the two large plasmids isolated from Xanthomonas axonopodis pv. citri, the agent of citrus canker and responsible for important economical losses in citrus world production. XACb0070 presents sequence homology only with other hypothetical proteins belonging to plant pathogens, none of which have their structure determined. The NMR-derived solution structure reveals this protein is a homodimer in which each monomer presents two domains with different structural and dynamic properties: a folded N-terminal domain with beta alpha alpha topology which mediates dimerization and a long disordered C-terminal tail. The folded domain shows high structural similarity to the ribbon-helix-helix transcriptional repressors, a family of DNA-binding proteins of conserved 3D fold but low sequence homology: indeed XACb0070 binds DNA. Primary sequence and fold comparison of XACb0070 with other proteins of the ribbon-helix-helix family together with examination of the genes in the vicinity of xacb0070 suggest the protein might be the component of a toxin-antitoxin system.
XACb0070 is an uncharacterized protein coded by the two large plasmids isolated from Xanthomonas axonopodis pv. citri, the agent of citrus canker and responsible for important economical losses in citrus world production. XACb0070 presents sequence homology only with other hypothetical proteins belonging to plant pathogens, none of which have their structure determined. The NMR-derived solution structure reveals this protein is a homodimer in which each monomer presents two domains with different structural and dynamic properties: a folded N-terminal domain with βαα topology which mediates dimerization and a long disordered C-terminal tail. The folded domain shows high structural similarity to the ribbon-helix-helix transcriptional repressors, a family of DNA-binding proteins of conserved 3D fold but low sequence homology: indeed XACb0070 binds DNA. Primary sequence and fold comparison of XACb0070 with other proteins of the ribbon-helix-helix family together with examination of the genes in the vicinity of xacb0070 suggest the protein might be the component of a toxin–antitoxin system.
Author Eliseo, Tommaso
Spisni, Alberto
Gallo, Mariana
Amata, Irene
Ferrari, Elena
Pertinhez, Thelma A.
Paci, Maurizio
Katsuyama, Angela M.
Farah, Chuck S.
Cicero, Daniel O.
Author_xml – sequence: 1
  givenname: Mariana
  surname: Gallo
  fullname: Gallo, Mariana
  organization: Department of Chemical Science and Technology, University of Rome “Tor Vergata”, Rome 00133, Italy
– sequence: 2
  givenname: Elena
  surname: Ferrari
  fullname: Ferrari, Elena
  organization: Department of Experimental Medicine, University of Parma, Italy
– sequence: 3
  givenname: Tommaso
  surname: Eliseo
  fullname: Eliseo, Tommaso
  organization: Department of Chemical Science and Technology, University of Rome “Tor Vergata”, Rome 00133, Italy
– sequence: 4
  givenname: Irene
  surname: Amata
  fullname: Amata, Irene
  organization: Department of Chemical Science and Technology, University of Rome “Tor Vergata”, Rome 00133, Italy
– sequence: 5
  givenname: Thelma A.
  surname: Pertinhez
  fullname: Pertinhez, Thelma A.
  organization: Department of Experimental Medicine, University of Parma, Italy
– sequence: 6
  givenname: Angela M.
  surname: Katsuyama
  fullname: Katsuyama, Angela M.
  organization: Department of Biochemistry, University of São Paulo, Brazil
– sequence: 7
  givenname: Maurizio
  surname: Paci
  fullname: Paci, Maurizio
  organization: Department of Chemical Science and Technology, University of Rome “Tor Vergata”, Rome 00133, Italy
– sequence: 8
  givenname: Chuck S.
  surname: Farah
  fullname: Farah, Chuck S.
  organization: Department of Biochemistry, University of São Paulo, Brazil
– sequence: 9
  givenname: Alberto
  surname: Spisni
  fullname: Spisni, Alberto
  email: alberto.spisni@unipr.it
  organization: Department of Experimental Medicine, University of Parma, Italy
– sequence: 10
  givenname: Daniel O.
  surname: Cicero
  fullname: Cicero, Daniel O.
  email: cicero@scienze.uniroma2.it
  organization: Department of Chemical Science and Technology, University of Rome “Tor Vergata”, Rome 00133, Italy
BackLink https://www.ncbi.nlm.nih.gov/pubmed/20060909$$D View this record in MEDLINE/PubMed
BookMark eNo1kU1r3DAQhkXZ0Hy0P6CXoltPdvVlyaKnJTRNIJBLArkJ2R53tdiSK2nT3VP-erTd9DIf8LzDOzOXaOWDB4S-UFJTQuX3bb1NXc0I0TVlNWHsA7qgRDdVKxu1OtZCVa1Q6hxdprQlhAjK6Ed0XiSSaKIv0Osae_iLZ5g7iDiMOG8AR9d1wVcbmNz-FHGO1qc-uiW74PFo-xwiTrsF4mhnNx3wGMP8T7xM1me82LwJv8Hj59Jtwhy8Tdjugw9LGFzCy0vduxzdJ3Q22inB5_d8hZ5ufj5e31b3D7_urtf3FVCidCXasWWytRq4orojUrRlXcWEVgJAcj4w3rRNw0ZFYJBUDGTUjDVKCA4Dl_wKfTvNXWL4s4OUzexSD1MxC2GXjOJcaaUkK-TXd3LXzTCYJbrZxoP5f7MC_DgBUPy-OIgm9Q58D4OL0GczBGcoMccPma0pHzpKtaHMFMv8DQuPhVU
ContentType Journal Article
Copyright 2010 Elsevier Inc.
(c) 2010 Elsevier Inc. All rights reserved.
Copyright_xml – notice: 2010 Elsevier Inc.
– notice: (c) 2010 Elsevier Inc. All rights reserved.
DBID CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1016/j.jsb.2009.12.022
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE

MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1095-8657
EndPage 31
ExternalDocumentID 20060909
S1047847709003499
Genre Journal Article
Comparative Study
GroupedDBID ---
--K
--M
-DZ
-~X
.55
.GJ
.~1
0R~
1B1
1RT
1~.
1~5
29L
3O-
4.4
457
4G.
53G
5GY
5RE
5VS
7-5
71M
85S
8P~
9JM
AACTN
AAEDT
AAEDW
AAIAV
AAIKJ
AAKOC
AALRI
AAOAW
AAQFI
AAQXK
AAXUO
ABFNM
ABFRF
ABGSF
ABJNI
ABMAC
ABUDA
ABXDB
ABYKQ
ACDAQ
ACGFO
ACGFS
ACRLP
ADBBV
ADEZE
ADFGL
ADMUD
ADUVX
AEBSH
AEFWE
AEHWI
AEKER
AENEX
AETEA
AFFNX
AFKWA
AFTJW
AFXIZ
AGHFR
AGRDE
AGUBO
AGYEJ
AHHHB
AHPSJ
AI.
AIEXJ
AIKHN
AITUG
AJBFU
AJOXV
ALMA_UNASSIGNED_HOLDINGS
AMFUW
AMRAJ
ASPBG
AVWKF
AXJTR
AZFZN
BKOJK
BLXMC
CAG
COF
CS3
DM4
DOVZS
DU5
EBS
EFBJH
EFLBG
EJD
EO8
EO9
EP2
EP3
F5P
FA8
FDB
FEDTE
FGOYB
FIRID
FNPLU
FYGXN
G-2
G-Q
GBLVA
HLW
HVGLF
HZ~
H~9
IH2
IHE
J1W
K-O
KOM
L7B
LG5
LX2
M41
MO0
MVM
N9A
O-L
O9-
OAUVE
OZT
P-8
P-9
PC.
Q38
R2-
RIG
RNS
ROL
RPZ
SBG
SDF
SDG
SDP
SES
SEW
SPCBC
SSU
SSZ
T5K
TWZ
UKR
UNMZH
VH1
WH7
WUQ
X7M
XJT
XOL
XPP
Y6R
ZA5
ZGI
ZKB
ZMT
ZU3
ZXP
~02
~G-
~KM
AAXKI
AFJKZ
AKRWK
CGR
CUY
CVF
ECM
EIF
NPM
7X8
ID FETCH-LOGICAL-e1079-48f8268a9e3719b0648022724974ee633d2358552f70ed614d0f92257443ed363
IEDL.DBID AIKHN
ISSN 1047-8477
IngestDate Fri Aug 16 11:43:14 EDT 2024
Sat Sep 28 07:58:38 EDT 2024
Fri Feb 23 02:28:16 EST 2024
IsPeerReviewed true
IsScholarly true
Issue 1
Keywords τci
Ribbon-helix-helix fold
Citrus canker
J
TA
NMR
DNA-binding protein
RHH
Rh
Antitoxin
CSI
Xaa
rmsd
Xac
Language English
License (c) 2010 Elsevier Inc. All rights reserved.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-e1079-48f8268a9e3719b0648022724974ee633d2358552f70ed614d0f92257443ed363
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMID 20060909
PQID 733797762
PQPubID 23479
PageCount 11
ParticipantIDs proquest_miscellaneous_733797762
pubmed_primary_20060909
elsevier_sciencedirect_doi_10_1016_j_jsb_2009_12_022
PublicationCentury 2000
PublicationDate April 2010
2010-Apr
20100401
PublicationDateYYYYMMDD 2010-04-01
PublicationDate_xml – month: 04
  year: 2010
  text: April 2010
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Journal of structural biology
PublicationTitleAlternate J Struct Biol
PublicationYear 2010
Publisher Elsevier Inc
Publisher_xml – name: Elsevier Inc
References Viles, Donne, Kroon, Prusiner, Cohen, Dyson, Wright (bib47) 2001; 40
Cuff, Clamp, Siddiqui, Finlay, Barton (bib14) 1998; 14
Gomis-Rüth, Solá, Acebo, Párraga, Guasch, Eritja, González, Espinosa, del Solar, Coll (bib23) 1998; 17
Jensen, Gerdes (bib27) 1995; 17
Galvão-Botton, Katsuyama, Guzzo, Almeida, Farah, Valente (bib20) 2003; 552
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer, Bax (bib17) 1995; 6
Wilkins, Grimshaw, Receveur, Dobson, Jones, Smith (bib48) 1999; 38
Ottiger, Bax (bib39) 1999; 13
Alegria, Souza, Andrade, Docena, Khater, Ramos, Da Silva, Farah (bib2) 2005; 187
Das (bib16) 2003; 5
Li, Zhang, Inouye, Ikura (bib34) 2008; 380
Junius, O’Donoghue, Nilges, King (bib30) 1996; 271
Augustus, Reardon, Spicer (bib5) 2009; 106
Jones, Wilkins, Smith, Dobson (bib29) 1997; 10
Clore, Gronenborn, Tjandra (bib12) 1998; 131
Lee, Revington, Arrowsmith, Kay (bib33) 1994; 350
Farrow, Zhang, Forman-Kay, Kay (bib18) 1995; 6
Breg, van Opheusden, Burgering, Boelens, Kaptein (bib7) 1990; 346
Cicero, Contessa, Pertinhez, Gallo, Katsuyama, Paci, Farah, Spisni (bib10) 2007; 67
Nadra, Eliseo, Mok, Almeida, Bycroft, Paci, de Prat-Gay, Cicero (bib38) 2004; 31
Rauman, Brown, Sauer (bib43) 1994; 4
van Bakel, Huynen, Wijmenga (bib45) 2004; 20
Gerdes, Christensen, Løbuer-Olesen (bib21) 2005; 3
Laskowski, Rullmannn, MacArthur, Kaptein, Thornton (bib32) 1996; 8
Cornilescu, Delaglio, Bax (bib13) 1999; 13
Pruvost, Boher, Brocherieux, Nicole, Chiroleu (bib42) 2002; 92
Johnson (bib28) 2004; 278
Wishart, Sykes (bib49) 1994; 239
Golovanov, Barillà, Golovanova, Hayes, Lian (bib22) 2003; 50
Zweckstetter, Bax (bib51) 2000; 122
Anderson, Sauer (bib3) 2003; 100
Holm, Park (bib25) 2000; 16
Kay, Torchia, Bax (bib31) 1989; 28
Schreiter, Drennan (bib44) 2007; 5
Chivers, Sauer (bib9) 1999; 8
Alegria, Docena, Khater, Ramos, Da Silva, Farah (bib1) 2004; 186
Ferrari, Tsay, Eggleston, Spisni, Chapman (bib19) 2004; 114
Janin (bib26) 1997; 28
Clore, Garrett (bib11) 1999; 121
Magnuson (bib35) 2007; 189
Zielenkiewicz, Ceglowski (bib50) 2005; 187
Bracken, Carr, Cavanagh, Palmer (bib6) 1999; 285
Penel, Hughes, Doig (bib41) 1999; 287
Arcus, Rainey, Turner (bib4) 2005; 13
da Silva, Ferro, Reinach, Farah, Furlan, Quaggio (bib15) 2002; 17
Mittenhuber (bib37) 1999; 1
Mattison, Wilbur, So, Brennan (bib36) 2006; 281
Pandey, Gerdes (bib40) 2005; 33
Vauterin, Hoste, Kersters, Swings (bib46) 1995; 45
Hansen, Mueller, Pardi (bib24) 1998; 5
Cherny, Gazit (bib8) 2004; 279
References_xml – volume: 106
  start-page: 5065
  year: 2009
  end-page: 5069
  ident: bib5
  article-title: MetJ repressor interactions with DNA probed by in-cell NMR
  publication-title: Proc. Natl. Acad. Sci. USA
  contributor:
    fullname: Spicer
– volume: 281
  start-page: 37942
  year: 2006
  end-page: 37951
  ident: bib36
  article-title: Structure of FitAB from
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Brennan
– volume: 239
  start-page: 363
  year: 1994
  end-page: 392
  ident: bib49
  article-title: Chemical shifts as a tool for structure determination
  publication-title: Methods Enzymol.
  contributor:
    fullname: Sykes
– volume: 552
  start-page: 207
  year: 2003
  end-page: 213
  ident: bib20
  article-title: High-throughput screening of structural proteomics targets using NMR
  publication-title: FEBS Lett.
  contributor:
    fullname: Valente
– volume: 189
  start-page: 6089
  year: 2007
  end-page: 6092
  ident: bib35
  article-title: Hypothetical functions of the toxin–antitoxin system
  publication-title: J. Bacteriol.
  contributor:
    fullname: Magnuson
– volume: 287
  start-page: 127
  year: 1999
  end-page: 143
  ident: bib41
  article-title: Side-chain structures in the first turn of the alpha-helix
  publication-title: J. Mol. Biol.
  contributor:
    fullname: Doig
– volume: 346
  start-page: 586
  year: 1990
  end-page: 589
  ident: bib7
  article-title: Structure of Arc repressor in solution: evidence for a family of beta-sheet DNA-binding proteins
  publication-title: Nature
  contributor:
    fullname: Kaptein
– volume: 186
  start-page: 6186
  year: 2004
  end-page: 6197
  ident: bib1
  article-title: New protein–protein interactions identified for the regulatory and structural components and substrates of the type III secretion system of the phytopathogen
  publication-title: J. Bacteriol.
  contributor:
    fullname: Farah
– volume: 131
  start-page: 159
  year: 1998
  end-page: 162
  ident: bib12
  article-title: Direct structure refinement against residual dipolar couplings in the presence of rhombicity of unknown magnitude
  publication-title: J. Magn. Reson.
  contributor:
    fullname: Tjandra
– volume: 121
  start-page: 9008
  year: 1999
  end-page: 9012
  ident: bib11
  article-title: -factor, free
  publication-title: J. Am. Chem. Soc.
  contributor:
    fullname: Garrett
– volume: 17
  start-page: 205
  year: 1995
  end-page: 210
  ident: bib27
  article-title: Programmed cell death in bacteria: proteic plasmid stabilization systems
  publication-title: Mol. Microbiol.
  contributor:
    fullname: Gerdes
– volume: 279
  start-page: 8252
  year: 2004
  end-page: 8261
  ident: bib8
  article-title: The YefM antitoxin defines a family of natively unfolded proteins: implications as a novel antibacterial target
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Gazit
– volume: 114
  start-page: 341
  year: 2004
  end-page: 346
  ident: bib19
  article-title: Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1
  publication-title: J. Allergy Clin. Immunol.
  contributor:
    fullname: Chapman
– volume: 13
  start-page: 187
  year: 1999
  end-page: 191
  ident: bib39
  article-title: Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values
  publication-title: J. Mol. Biol.
  contributor:
    fullname: Bax
– volume: 40
  start-page: 2743
  year: 2001
  end-page: 2753
  ident: bib47
  article-title: Local structural plasticity of the prion protein. Analysis of NMR relaxation dynamics
  publication-title: Biochemistry
  contributor:
    fullname: Wright
– volume: 6
  start-page: 277
  year: 1995
  end-page: 293
  ident: bib17
  article-title: NMRPipe: a multidimensional spectral processing system based on UNIX pipes
  publication-title: J. Biomol. NMR
  contributor:
    fullname: Bax
– volume: 17
  start-page: 7404
  year: 1998
  end-page: 7415
  ident: bib23
  article-title: The structure of plasmid-encoded transcriptional repressor CopG unliganded and bound to its operator
  publication-title: EMBO J.
  contributor:
    fullname: Coll
– volume: 100
  start-page: 341
  year: 2003
  end-page: 350
  ident: bib3
  article-title: Role of an N
  publication-title: Biophys. Chem.
  contributor:
    fullname: Sauer
– volume: 14
  start-page: 892
  year: 1998
  end-page: 893
  ident: bib14
  article-title: Jpred: a consensus secondary structure prediction server
  publication-title: Bioinformatics
  contributor:
    fullname: Barton
– volume: 16
  start-page: 566
  year: 2000
  end-page: 567
  ident: bib25
  article-title: DaliLite workbench for protein structure comparison
  publication-title: Bioinformatics
  contributor:
    fullname: Park
– volume: 17
  start-page: 459
  year: 2002
  end-page: 463
  ident: bib15
  article-title: Comparison of the genomes of two
  publication-title: Nature
  contributor:
    fullname: Quaggio
– volume: 8
  start-page: 477
  year: 1996
  end-page: 486
  ident: bib32
  article-title: AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR
  publication-title: J. Biomol. NMR
  contributor:
    fullname: Thornton
– volume: 271
  start-page: 13663
  year: 1996
  end-page: 13667
  ident: bib30
  article-title: High resolution NMR solution structure of the leucine zipper domain of the c-Jun homodimer
  publication-title: J. Biol. Chem.
  contributor:
    fullname: King
– volume: 5
  start-page: 1065
  year: 1998
  end-page: 1074
  ident: bib24
  article-title: Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactions
  publication-title: Nat. Struct. Biol.
  contributor:
    fullname: Pardi
– volume: 38
  start-page: 16424
  year: 1999
  end-page: 16431
  ident: bib48
  article-title: Hydrodynamic radii of native and denatured proteins measured by pulse field gradient NMR techniques
  publication-title: Biochemistry
  contributor:
    fullname: Smith
– volume: 4
  start-page: 36
  year: 1994
  end-page: 43
  ident: bib43
  article-title: Major groove DNA recognition by β-sheets: the ribbon-helix-helix family of gene regulatory proteins
  publication-title: Curr. Opin. Struct. Biol.
  contributor:
    fullname: Sauer
– volume: 350
  start-page: 87
  year: 1994
  end-page: 90
  ident: bib33
  article-title: A pulsed field gradient isotope-filtered 3D 13C HMQC-NOESY experiment for extracting intermolecular NOE contacts in molecular complexes
  publication-title: FEBS Lett.
  contributor:
    fullname: Kay
– volume: 3
  start-page: 371
  year: 2005
  end-page: 382
  ident: bib21
  article-title: Prokaryotic toxin–antitoxin stress response loci
  publication-title: Nat. Rev. Microbiol.
  contributor:
    fullname: Løbuer-Olesen
– volume: 278
  start-page: 313
  year: 2004
  end-page: 352
  ident: bib28
  article-title: Using NMRView to visualize and analyze the NMR spectra of macromolecules
  publication-title: Methods Mol. Biol.
  contributor:
    fullname: Johnson
– volume: 285
  start-page: 2133
  year: 1999
  end-page: 2146
  ident: bib6
  article-title: Temperature dependence of intramolecular dynamics of the basic leucine zipper of GCN4: implications for the entropy of association with DNA
  publication-title: J. Mol. Biol.
  contributor:
    fullname: Palmer
– volume: 187
  start-page: 6094
  year: 2005
  end-page: 6105
  ident: bib50
  article-title: Toxin–antitoxin system of Streptococcal plasmid pSM19035
  publication-title: J. Bacteriol.
  contributor:
    fullname: Ceglowski
– volume: 10
  start-page: 199
  year: 1997
  end-page: 203
  ident: bib29
  article-title: Characterisation of protein unfolding by NMR diffusion measurements
  publication-title: J. Biomol. NMR
  contributor:
    fullname: Dobson
– volume: 33
  start-page: 966
  year: 2005
  end-page: 976
  ident: bib40
  article-title: Toxin–antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes
  publication-title: Nucleic Acids Res.
  contributor:
    fullname: Gerdes
– volume: 5
  start-page: 710
  year: 2007
  end-page: 720
  ident: bib44
  article-title: Ribbon-helix-helix transcription factors: variations on a theme
  publication-title: Nat. Rev. Microbiol.
  contributor:
    fullname: Drennan
– volume: 31
  start-page: 211
  year: 2004
  end-page: 214
  ident: bib38
  article-title: Solution structure of the HPV-16 E2 DNA binding domain, a transcriptional regulator with a dimeric β-barrel fold
  publication-title: J. Biomol. NMR
  contributor:
    fullname: Cicero
– volume: 67
  start-page: 490
  year: 2007
  end-page: 500
  ident: bib10
  article-title: Solution Structure of ApaG from
  publication-title: Proteins
  contributor:
    fullname: Spisni
– volume: 50
  start-page: 1141
  year: 2003
  end-page: 1153
  ident: bib22
  article-title: ParG, a protein required for active partition of bacterial plasmids, has a dimeric ribbon-helix-helix structure
  publication-title: Mol. Microbiol.
  contributor:
    fullname: Lian
– volume: 1
  start-page: 295
  year: 1999
  end-page: 302
  ident: bib37
  article-title: Occurrence of mazEF-like antitoxin/toxin systems in bacteria
  publication-title: J. Mol. Microbiol. Biotechnol.
  contributor:
    fullname: Mittenhuber
– volume: 13
  start-page: 289
  year: 1999
  end-page: 302
  ident: bib13
  article-title: Protein backbone angle restraints from searching a database for chemical shift and sequence homology
  publication-title: J. Biomol. NMR
  contributor:
    fullname: Bax
– volume: 8
  start-page: 2494
  year: 1999
  end-page: 2500
  ident: bib9
  article-title: NikR is a ribbon-helix-helix DNA-binding protein
  publication-title: Protein Sci.
  contributor:
    fullname: Sauer
– volume: 5
  start-page: 52
  year: 2003
  end-page: 60
  ident: bib16
  article-title: Citrus canker – a review
  publication-title: J. Appl. Hort.
  contributor:
    fullname: Das
– volume: 6
  start-page: 153
  year: 1995
  end-page: 162
  ident: bib18
  article-title: Spectral density function mapping using
  publication-title: J. Biomol. NMR
  contributor:
    fullname: Kay
– volume: 187
  start-page: 2315
  year: 2005
  end-page: 2325
  ident: bib2
  article-title: Identification of new protein–protein interactions involving the products of the chromosome- and plasmid-encoded type IV secretion loci of the phytopathogen
  publication-title: J. Bacteriol.
  contributor:
    fullname: Farah
– volume: 13
  start-page: 360
  year: 2005
  end-page: 365
  ident: bib4
  article-title: The PIN-domain toxin–antitoxin array in mycobacteria
  publication-title: Trends Microbiol.
  contributor:
    fullname: Turner
– volume: 45
  start-page: 472
  year: 1995
  end-page: 489
  ident: bib46
  article-title: Reclassification of
  publication-title: Int. J. Syst. Bacteriol.
  contributor:
    fullname: Swings
– volume: 92
  start-page: 336
  year: 2002
  end-page: 346
  ident: bib42
  article-title: Survival of
  publication-title: Phytopathology
  contributor:
    fullname: Chiroleu
– volume: 20
  start-page: 2644
  year: 2004
  end-page: 2655
  ident: bib45
  article-title: Prokaryotic diversity of the
  publication-title: Bioinformatics
  contributor:
    fullname: Wijmenga
– volume: 122
  start-page: 3791
  year: 2000
  end-page: 3792
  ident: bib51
  article-title: Prediction of sterically induced alignment in a dilute liquid crystalline phase: aid to protein structure determination by NMR
  publication-title: J. Am. Chem. Soc.
  contributor:
    fullname: Bax
– volume: 28
  start-page: 8972
  year: 1989
  end-page: 8979
  ident: bib31
  article-title: Backbone dynamics of proteins as studied by
  publication-title: Biochemistry
  contributor:
    fullname: Bax
– volume: 28
  start-page: 153
  year: 1997
  end-page: 161
  ident: bib26
  article-title: The kinetics of protein–protein recognition
  publication-title: Prot. Struct. Funct. Genet.
  contributor:
    fullname: Janin
– volume: 380
  start-page: 107
  year: 2008
  end-page: 119
  ident: bib34
  article-title: Structural mechanism of transcriptional autorepression of the
  publication-title: J. Mol. Biol.
  contributor:
    fullname: Ikura
SSID ssj0004121
Score 1.9728549
Snippet XACb0070 is an uncharacterized protein coded by the two large plasmids isolated from Xanthomonas axonopodis pv. citri, the agent of citrus canker and...
SourceID proquest
pubmed
elsevier
SourceType Aggregation Database
Index Database
Publisher
StartPage 21
SubjectTerms Amino Acid Sequence
Antitoxin
Base Sequence
Citrus - microbiology
Citrus canker
Dimerization
DNA-binding protein
Electrophoresis, Polyacrylamide Gel
Models, Molecular
Molecular Sequence Data
NMR
Nuclear Magnetic Resonance, Biomolecular
Plasmids - genetics
Protein Conformation
Ribbon-helix-helix fold
Sequence Analysis, DNA
Spectrometry, Fluorescence
Transcription Factors - genetics
Xanthomonas axonopodis - genetics
Title A new member of the ribbon-helix-helix transcription factor superfamily from the plant pathogen Xanthomonas axonopodis pv.citri
URI https://dx.doi.org/10.1016/j.jsb.2009.12.022
https://www.ncbi.nlm.nih.gov/pubmed/20060909
https://search.proquest.com/docview/733797762
Volume 170
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB61WyFxQbxpgcoHrulu1t44Pq4qqoUVPQAVe7PieCxSlSTa7KL2An-dmTgRcODCJZGijGLNTOZhz8wH8MalZUGpzyJJUatE5QaTHEOZlEjex6sSvedE8cNltrpS7zeLzQGcj70wXFY52P5o03trPTyZDtyctlU1_dQPllFaz0w_ZMUcwhG5I6UmcLR8t15d_m6PTGP7FQ8lYILxcLMv87ruXJxayZuCDKH7h0_6V8zZ-56Lh_BgCBrFMq7rERxg_RjuRRjJuyfwcykoOBbfkME9RBMEBXViWznX1MlXvKlu41Xs2C-NVkJEpB3R7Vvcxm0Owb0mPXF7QwwXjFbckIKJTcEjBkhhi04Ut03dtI2vOtF-Pyur3bZ6ClcXbz-fr5IBWCFByvYMiSRQVpEXBqVOjaOohBtuNWViWiFmUnpuoF0s5kHP0JMD97Ng6MfXSkn0MpPPYFI3Nb4AkbNQ9cyl2ZydvctNmJdpCN7JIlcYjkGN_LR_ydWSybZjidm1JTEwIqax6dzSWo5BjLy3pO98iFHU2Ow7q6XUFLNm9MrzKBPbxrkcTJ-RMpiT__vmS7gfSwO4LOcVTHbbPb6miGPnTuHw7Ed6OugV39cfv6x_AZQS16E
link.rule.ids 315,786,790,4521,24144,27955,27956,45618,45712
linkProvider Elsevier
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwELYoCJVLVR4t0BZ84Bp2s_bG8RGhoqU8LoC0NyuOxyKIJtFmF8EF_jozcSLKgUsvOUS2YnkmM9_Y38wwdmDjPMPQZxzFoGQkUw1RCj6PckDv42QOzlGgeHGZTG7kn-l4usSO-1wYolV2tj_Y9NZad28G3W4O6qIYXLWFZaRSQ90WWdGf2AqhAeJ1HT6_8TxkHJKvqCQBDe-vNluS111jQ81KOhKkBrr_eKSPEGfreU6-si8dZORHYVXrbAnKDbYamkg-bbKXI47QmP8Fau3BK88R0vFZYW1VRrdwXzyGJ5-TV-ptBA99dnizqGEWDjk4ZZq0k-t73G5OvYorVC8-zajAAKpr1vDssSqrunJFw-uHw7yYz4otdnPy-_p4EnVtFSLAWE-jQDzGFGmmQahYW8QklG6rMA5TEiARwlH67Hg88moIDt23G3qNv72SUoATifjGlsuqhG3GUxKpGto4GZGrt6n2ozz23lmRpRL8DpP9fpp3UjVosE1PMLszKAbqh6lNPDK4lh3G-703qO10hZGVUC0ao4RQiFgTHPI9yMTUoSoHzU9QFfTu_31zn32eXF-cm_PTy7MfbC2QBIig85Mtz2cL-IXYY273Wt16BcHN1tM
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+new+member+of+the+ribbon-helix-helix+transcription+factor+superfamily+from+the+plant+pathogen+Xanthomonas+axonopodis+pv.citri&rft.jtitle=Journal+of+structural+biology&rft.au=Gallo%2C+Mariana&rft.au=Ferrari%2C+Elena&rft.au=Eliseo%2C+Tommaso&rft.au=Amata%2C+Irene&rft.date=2010-04-01&rft.eissn=1095-8657&rft.volume=170&rft.issue=1&rft.spage=21&rft.epage=31&rft_id=info:doi/10.1016%2Fj.jsb.2009.12.022&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1047-8477&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1047-8477&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1047-8477&client=summon