Strategic Sampling of Eurasian Otter Spraints for Genetic Research in South Korea: Enhancing PCR Success and Data Accuracy

• Published in: Animals (Basel) Vol. 15; no. 4; p. 574
• Main Authors: Kim, Jee Hyun, Lee, Jangmi, Kim, Dong Youn, Yang, Yoon-Do, Cho, Sujoo, Park, Han-Chan, Han, Sung Yong, Min, Mi-Sook, Lee, Hang, Cho, Je-Yoel, Pandey, Puneet
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.02.2025; MDPI
• Subjects: Defecation; DNA degradation; Endangered & extinct species; Endangered species; Ethanol; Eurasian otter; Feces; Genetic research; genotyping errors; Humidity; non-invasive sampling; Otters; PCR amplification success; Success; Temperature; Winter; South Korea; Germany; United States > US; PCR amplification success; non-invasive sampling; DNA degradation; genotyping errors; Eurasian otter

Non-invasive genetic approaches, particularly using fecal samples, are commonly used to study endangered and elusive species, as they are easy to collect with minimal permission and cause little disturbance to the subject population. However, such studies face limitations due to poor DNA yield, whic...