Exploring the virulence gene interactome with CRISPR/dCas9 in the human malaria parasite

• Published in: Molecular systems biology Vol. 16; no. 8; pp. e9569 - n/a
• Main Authors: Bryant, Jessica M, Baumgarten, Sebastian, Dingli, Florent, Loew, Damarys, Sinha, Ameya, Claës, Aurélie, Preiser, Peter R, Dedon, Peter C, Scherf, Artur
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.08.2020; EMBO Press; John Wiley and Sons Inc; Springer Nature
• Subjects: Adenosine Triphosphatases - metabolism; Animals; Antigens, Protozoan - genetics; Antigens, Protozoan - metabolism; Chromatin; Chromatin Immunoprecipitation Sequencing; Chromosomes; CRISPR; CRISPR-Cas Systems; Deoxyribonucleic acid; Divergence; DNA; EMBO09; EMBO23; Enrichment; Epigenetics; Erythrocytes; Genes; Genomes; Genomics; Humans; Immune system; Introns; Life Sciences; Malaria; Mass Spectrometry; Mass spectroscopy; Microbiology and Parasitology; MORC protein; Parasites; Parasitology; Pathogenesis; Plasmodium falciparum; Plasmodium falciparum - genetics; Plasmodium falciparum - immunology; Plasmodium falciparum - pathogenicity; Promoter Regions, Genetic; Protein Interaction Maps; Proteins; Proteomics; Proteomics - methods; Regulatory sequences; Structural proteins; Transcription activation; Transcription factors; Transcription Factors - metabolism; Var gene; var genes; Vector-borne diseases; Virulence; Virulence Factors - genetics; Virulence Factors - metabolism; CRISPR; chromatin; genes; epigenetics; Plasmodium falciparum; var genes; Virology & Host Pathogen Interaction; Microbiology; var genes Subject Categories Chromatin; Transcription & Genomics

Mutually exclusive expression of the var multigene family is key to immune evasion and pathogenesis in Plasmodium falciparum , but few factors have been shown to play a direct role. We adapted a CRISPR‐based proteomics approach to identify novel factors associated with var genes in their natural chr...