RNA Surveillance Factor SMG5 Is Essential for Mouse Embryonic Stem Cell Differentiation
Nonsense-mediated mRNA decay (NMD) is a highly conserved post-transcriptional gene expression regulatory mechanism in eukaryotic cells. NMD eliminates aberrant mRNAs with premature termination codons to surveil transcriptome integrity. Furthermore, NMD fine-tunes gene expression by destabilizing RNA...
Saved in:
| Published in | Biomolecules (Basel, Switzerland) Vol. 14; no. 8; p. 1023 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Switzerland
MDPI AG
01.08.2024
MDPI |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Nonsense-mediated mRNA decay (NMD) is a highly conserved post-transcriptional gene expression regulatory mechanism in eukaryotic cells. NMD eliminates aberrant mRNAs with premature termination codons to surveil transcriptome integrity. Furthermore, NMD fine-tunes gene expression by destabilizing RNAs with specific NMD features. Thus, by controlling the quality and quantity of the transcriptome, NMD plays a vital role in mammalian development, stress response, and tumorigenesis. Deficiencies of NMD factors result in early embryonic lethality, while the underlying mechanisms are poorly understood. SMG5 is a key NMD factor. In this study, we generated an
conditional knockout mouse model and found that
-null results in early embryonic lethality before E13.5. Furthermore, we produced multiple lines of
knockout mouse embryonic stem cells (mESCs) and found that the deletion of
in mESCs does not compromise cell viability.
-null delays differentiation of mESCs. Mechanistically, our study reveals that the c-MYC protein, but not
mRNA, is upregulated in SMG5-deficient mESCs. The overproduction of c-MYC protein could be caused by enhanced protein synthesis upon SMG5 loss. Furthermore, SMG5-null results in dysregulation of alternative splicing on multiple stem cell differentiation regulators. Overall, our findings underscore the importance of SMG5-NMD in regulating mESC cell-state transition. |
|---|---|
| AbstractList | Nonsense-mediated mRNA decay (NMD) is a highly conserved post-transcriptional gene expression regulatory mechanism in eukaryotic cells. NMD eliminates aberrant mRNAs with premature termination codons to surveil transcriptome integrity. Furthermore, NMD fine-tunes gene expression by destabilizing RNAs with specific NMD features. Thus, by controlling the quality and quantity of the transcriptome, NMD plays a vital role in mammalian development, stress response, and tumorigenesis. Deficiencies of NMD factors result in early embryonic lethality, while the underlying mechanisms are poorly understood. SMG5 is a key NMD factor. In this study, we generated an Smg5 conditional knockout mouse model and found that Smg5-null results in early embryonic lethality before E13.5. Furthermore, we produced multiple lines of Smg5 knockout mouse embryonic stem cells (mESCs) and found that the deletion of Smg5 in mESCs does not compromise cell viability. Smg5-null delays differentiation of mESCs. Mechanistically, our study reveals that the c-MYC protein, but not c-Myc mRNA, is upregulated in SMG5-deficient mESCs. The overproduction of c-MYC protein could be caused by enhanced protein synthesis upon SMG5 loss. Furthermore, SMG5-null results in dysregulation of alternative splicing on multiple stem cell differentiation regulators. Overall, our findings underscore the importance of SMG5-NMD in regulating mESC cell-state transition. Nonsense-mediated mRNA decay (NMD) is a highly conserved post-transcriptional gene expression regulatory mechanism in eukaryotic cells. NMD eliminates aberrant mRNAs with premature termination codons to surveil transcriptome integrity. Furthermore, NMD fine-tunes gene expression by destabilizing RNAs with specific NMD features. Thus, by controlling the quality and quantity of the transcriptome, NMD plays a vital role in mammalian development, stress response, and tumorigenesis. Deficiencies of NMD factors result in early embryonic lethality, while the underlying mechanisms are poorly understood. SMG5 is a key NMD factor. In this study, we generated an Smg5 conditional knockout mouse model and found that Smg5-null results in early embryonic lethality before E13.5. Furthermore, we produced multiple lines of Smg5 knockout mouse embryonic stem cells (mESCs) and found that the deletion of Smg5 in mESCs does not compromise cell viability. Smg5-null delays differentiation of mESCs. Mechanistically, our study reveals that the c-MYC protein, but not c-Myc mRNA, is upregulated in SMG5-deficient mESCs. The overproduction of c-MYC protein could be caused by enhanced protein synthesis upon SMG5 loss. Furthermore, SMG5-null results in dysregulation of alternative splicing on multiple stem cell differentiation regulators. Overall, our findings underscore the importance of SMG5-NMD in regulating mESC cell-state transition.Nonsense-mediated mRNA decay (NMD) is a highly conserved post-transcriptional gene expression regulatory mechanism in eukaryotic cells. NMD eliminates aberrant mRNAs with premature termination codons to surveil transcriptome integrity. Furthermore, NMD fine-tunes gene expression by destabilizing RNAs with specific NMD features. Thus, by controlling the quality and quantity of the transcriptome, NMD plays a vital role in mammalian development, stress response, and tumorigenesis. Deficiencies of NMD factors result in early embryonic lethality, while the underlying mechanisms are poorly understood. SMG5 is a key NMD factor. In this study, we generated an Smg5 conditional knockout mouse model and found that Smg5-null results in early embryonic lethality before E13.5. Furthermore, we produced multiple lines of Smg5 knockout mouse embryonic stem cells (mESCs) and found that the deletion of Smg5 in mESCs does not compromise cell viability. Smg5-null delays differentiation of mESCs. Mechanistically, our study reveals that the c-MYC protein, but not c-Myc mRNA, is upregulated in SMG5-deficient mESCs. The overproduction of c-MYC protein could be caused by enhanced protein synthesis upon SMG5 loss. Furthermore, SMG5-null results in dysregulation of alternative splicing on multiple stem cell differentiation regulators. Overall, our findings underscore the importance of SMG5-NMD in regulating mESC cell-state transition. Nonsense-mediated mRNA decay (NMD) is a highly conserved post-transcriptional gene expression regulatory mechanism in eukaryotic cells. NMD eliminates aberrant mRNAs with premature termination codons to surveil transcriptome integrity. Furthermore, NMD fine-tunes gene expression by destabilizing RNAs with specific NMD features. Thus, by controlling the quality and quantity of the transcriptome, NMD plays a vital role in mammalian development, stress response, and tumorigenesis. Deficiencies of NMD factors result in early embryonic lethality, while the underlying mechanisms are poorly understood. SMG5 is a key NMD factor. In this study, we generated an conditional knockout mouse model and found that -null results in early embryonic lethality before E13.5. Furthermore, we produced multiple lines of knockout mouse embryonic stem cells (mESCs) and found that the deletion of in mESCs does not compromise cell viability. -null delays differentiation of mESCs. Mechanistically, our study reveals that the c-MYC protein, but not mRNA, is upregulated in SMG5-deficient mESCs. The overproduction of c-MYC protein could be caused by enhanced protein synthesis upon SMG5 loss. Furthermore, SMG5-null results in dysregulation of alternative splicing on multiple stem cell differentiation regulators. Overall, our findings underscore the importance of SMG5-NMD in regulating mESC cell-state transition. Nonsense-mediated mRNA decay (NMD) is a highly conserved post-transcriptional gene expression regulatory mechanism in eukaryotic cells. NMD eliminates aberrant mRNAs with premature termination codons to surveil transcriptome integrity. Furthermore, NMD fine-tunes gene expression by destabilizing RNAs with specific NMD features. Thus, by controlling the quality and quantity of the transcriptome, NMD plays a vital role in mammalian development, stress response, and tumorigenesis. Deficiencies of NMD factors result in early embryonic lethality, while the underlying mechanisms are poorly understood. SMG5 is a key NMD factor. In this study, we generated an Smg5 conditional knockout mouse model and found that Smg5 -null results in early embryonic lethality before E13.5. Furthermore, we produced multiple lines of Smg5 knockout mouse embryonic stem cells (mESCs) and found that the deletion of Smg5 in mESCs does not compromise cell viability. Smg5 -null delays differentiation of mESCs. Mechanistically, our study reveals that the c-MYC protein, but not c-Myc mRNA, is upregulated in SMG5-deficient mESCs. The overproduction of c-MYC protein could be caused by enhanced protein synthesis upon SMG5 loss. Furthermore, SMG5-null results in dysregulation of alternative splicing on multiple stem cell differentiation regulators. Overall, our findings underscore the importance of SMG5-NMD in regulating mESC cell-state transition. |
| Audience | Academic |
| Author | Jiang, Xiaoning Chen, Chengyan Wei, Yanling Li, Tangliang |
| AuthorAffiliation | 2 School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou 311121, China 1 State Key Laboratory of Microbial Technology, Shandong University, Qingdao Campus, Qingdao 266237, China |
| AuthorAffiliation_xml | – name: 1 State Key Laboratory of Microbial Technology, Shandong University, Qingdao Campus, Qingdao 266237, China – name: 2 School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou 311121, China |
| Author_xml | – sequence: 1 givenname: Chengyan surname: Chen fullname: Chen, Chengyan organization: State Key Laboratory of Microbial Technology, Shandong University, Qingdao Campus, Qingdao 266237, China – sequence: 2 givenname: Yanling surname: Wei fullname: Wei, Yanling organization: School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou 311121, China – sequence: 3 givenname: Xiaoning surname: Jiang fullname: Jiang, Xiaoning organization: School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou 311121, China – sequence: 4 givenname: Tangliang orcidid: 0000-0003-0671-9166 surname: Li fullname: Li, Tangliang organization: School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou 311121, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/39199410$$D View this record in MEDLINE/PubMed |
| BookMark | eNptUs1rFDEcDVKxtfbmWQJevGzN1-TjJMu6rQutgqvoLWQyyZplZtJmZgr97_1tW2VXTEIS3u_lJe-Rl-ioz31A6DUl55wb8r5OuaOCaEoYf4ZOGKN6xhT_ebS3P0Znw7Al0DQMxl-gY26oMYKSE_Tj6-c5Xk_lLqS2db0P-ML5MRe8vr6s8GrAy2EI_ZhciyOg13kaAl52dbnPffJ4PYYOL0Lb4o8pxlAeqGPK_Sv0PLp2CGdP6yn6frH8tvg0u_pyuVrMr2YNr_Q4oyY0VDoHs9ZCB6NkbRwRgXhFOdVKiEYZqmrpInGN2BVl0NxI4SnXjp-i1aNuk93W3pTUuXJvs0v2AchlY10Zk2-D1Z4p1VSUReFFBA0mvZGykSY4gCJofXjUupnqLjQezBTXHogeVvr0y27ynaWUV0xyDgrvnhRKvp3CMNouDT7skg2QnOXEGCoYkxqob_-hbvNUeshqx1LwPG72WBsHDlIfM1zsd6J2rgmkw1QlgHX-Hxb0JnTJw4-JCfCDA2_2nf61-Odf8N9E-Ll- |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2024 MDPI AG 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2024 by the authors. 2024 |
| Copyright_xml | – notice: COPYRIGHT 2024 MDPI AG – notice: 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2024 by the authors. 2024 |
| DBID | CGR CUY CVF ECM EIF NPM 3V. 7T5 7TM 7TO 7X7 7XB 88E 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ H94 HCIFZ K9. LK8 M0S M1P M7P PIMPY PQEST PQQKQ PQUKI PRINS 7X8 5PM DOA |
| DOI | 10.3390/biom14081023 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Immunology Abstracts Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central ProQuest Central Essentials Biological Science Collection AUTh Library subscriptions: ProQuest Central ProQuest Natural Science Collection ProQuest One Community College ProQuest Central Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection (Proquest) (PQ_SDU_P3) ProQuest Health & Medical Complete (Alumni) Biological Sciences Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Biological Science Database Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest Central Student Oncogenes and Growth Factors Abstracts ProQuest Central Essentials Nucleic Acids Abstracts ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Central China ProQuest Central Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Biological Science Collection AIDS and Cancer Research Abstracts ProQuest Medical Library (Alumni) ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition Immunology Abstracts ProQuest One Academic ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | Publicly Available Content Database MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: DOA name: Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: BENPR name: AUTh Library subscriptions: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology |
| EISSN | 2218-273X |
| ExternalDocumentID | oai_doaj_org_article_8c277d512f4c4f83926c966d69eaf4cf A807442754 39199410 |
| Genre | Journal Article |
| GeographicLocations | China Grand Island United States--US |
| GeographicLocations_xml | – name: China – name: Grand Island – name: United States--US |
| GrantInformation_xml | – fundername: Natural Science Foundation of Zhejiang Province grantid: LY22C050003 – fundername: Natural Science Foundation of Shandong Province grantid: ZR2023MC013 – fundername: Natural Science Foundation of Shandong Province and the Qilu Youth Scholar Startup Funding of Shandong University grantid: ZR2023MC013 |
| GroupedDBID | 3V. 53G 5VS 7X7 88E 8FE 8FH 8FI 8FJ AADQD AAFWJ ABDBF ABUWG ADBBV AFKRA AFPKN AFZYC ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI CCPQU CGR CUY CVF EBD ECM EIF ESX FYUFA GROUPED_DOAJ HCIFZ HMCUK HYE IAO ITC KQ8 LK8 M1P M48 M7P MODMG M~E NPM OK1 PGMZT PIMPY PQQKQ PROAC PSQYO RIG RPM UKHRP 7T5 7TM 7TO 7XB 8FK AZQEC DWQXO GNUQQ H94 K9. PQEST PQUKI PRINS 7X8 5PM |
| ID | FETCH-LOGICAL-d358t-19ed16aaed18848e976b9a04e0c71318744d7917b6af0ad4b9a06e83964c138a3 |
| IEDL.DBID | RPM |
| ISSN | 2218-273X |
| IngestDate | Tue Oct 22 15:16:21 EDT 2024 Fri Aug 30 06:00:13 EDT 2024 Sat Oct 26 01:58:30 EDT 2024 Thu Oct 10 22:00:18 EDT 2024 Thu Sep 12 00:37:05 EDT 2024 Tue Sep 10 03:51:20 EDT 2024 Tue Oct 29 09:25:01 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 8 |
| Keywords | differentiation mouse embryonic stem cells nonsense-mediated mRNA decay c-MYC SMG5 |
| Language | English |
| License | Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-d358t-19ed16aaed18848e976b9a04e0c71318744d7917b6af0ad4b9a06e83964c138a3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0003-0671-9166 |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11352633/ |
| PMID | 39199410 |
| PQID | 3097839398 |
| PQPubID | 2032425 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_8c277d512f4c4f83926c966d69eaf4cf pubmedcentral_primary_oai_pubmedcentral_nih_gov_11352633 proquest_miscellaneous_3099142268 proquest_journals_3097839398 gale_infotracmisc_A807442754 gale_infotracacademiconefile_A807442754 pubmed_primary_39199410 |
| PublicationCentury | 2000 |
| PublicationDate | 2024-08-01 |
| PublicationDateYYYYMMDD | 2024-08-01 |
| PublicationDate_xml | – month: 08 year: 2024 text: 2024-08-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | Switzerland |
| PublicationPlace_xml | – name: Switzerland – name: Basel |
| PublicationTitle | Biomolecules (Basel, Switzerland) |
| PublicationTitleAlternate | Biomolecules |
| PublicationYear | 2024 |
| Publisher | MDPI AG MDPI |
| Publisher_xml | – name: MDPI AG – name: MDPI |
| SSID | ssj0000800823 |
| Score | 2.3509097 |
| Snippet | Nonsense-mediated mRNA decay (NMD) is a highly conserved post-transcriptional gene expression regulatory mechanism in eukaryotic cells. NMD eliminates aberrant... |
| SourceID | doaj pubmedcentral proquest gale pubmed |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database |
| StartPage | 1023 |
| SubjectTerms | Alternative splicing Animals c-MYC c-Myc protein Cell differentiation Cell Differentiation - genetics Cell viability Clonal deletion Cloning Codons differentiation Embryo cells Embryogenesis Embryonic stem cells Embryos Enzymes Ethylenediaminetetraacetic acid Gene expression Genes Lethality Messenger RNA Mice Mice, Knockout mouse embryonic stem cells Mouse Embryonic Stem Cells - cytology Mouse Embryonic Stem Cells - metabolism mRNA turnover Myc protein Neomycin Nonsense Mediated mRNA Decay - genetics Nonsense-mediated mRNA decay Post-transcription Protein biosynthesis Proto-Oncogene Proteins c-myc - genetics Proto-Oncogene Proteins c-myc - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism SMG5 Stem cells Transcription termination Transcriptomes Tumorigenesis |
| SummonAdditionalLinks | – databaseName: Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV07T8MwELYQEwsCyiO8ZCQEU0QSO449Fmh5SDBQEGyR7bgC1AbUBqT-e-6cgBoxsLBksB3JufPj-6K77wg55MDWgKOZMHLchID_RSjjmIU2tlhRRBSZ8VG-t-LygV8_pU9zpb4wJqyWB64NdyJtkmUFXEtDbvkQr3NhAaIXQjkNTUN_-kZqjky9NjhIJqyOdGfA608wmx3IhESpgkaf__cpPHcNtUMk5-6c_gpZbsAi7daTXCULrlwjnW4JRHk8o0fUh2_6_-Id8nh326WDj8mnwzpC4Era96V06ODmIqVXU9qbYp4RLDcKOJXeAON3tDc2kxmK49JB5cb0zI1G9LwpmVLVTlsnD_3e_dll2FRNCAuWyiqMlStioTU8peTSAd4wSkfcRRYIKZbg40UGJM0IPYx0wbFTODCs4DZmUrMNsli-lW6L0CQSWkKTVcZx5pQyCRMyAkwH3FMkOiCnaMf8vRbGyFGq2jeAA_PGgflfDgzIMXohxw0Fpra6yQuAKaA0Vd5FuR6eZCkPyG5rJGwE2-7-9mPebMRpznyeimJKBuTgpxvfxOCy0oGxcYzyGcUwZrN2-88nMYXiyXEUENlaEK1vbveUL89ephuWfJoIxrb_w0o7ZCkBOFWHHu6SxWry4fYADlVm36_8Ly-CB3o priority: 102 providerName: Directory of Open Access Journals – databaseName: AUTh Library subscriptions: ProQuest Central dbid: BENPR link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Nb9QwEB3B9sIFFcpHoCAjIThFTWzHsU9oW3YpSF2hLhW9RY7jBaRutt1NkfrvmXHcpRESlxxsR0o8Y-c9Z-YNwFuJbA05Wp1mXtYp4n-V6jwXqcsdVRRRTVmHKN-ZOj6TX86L83jgtolhlbd7Ytiom5WjM_IDERIOjDD6w-VVSlWj6O9qLKFxH3Y4MgU-gp3Dyezr6faUhfCQ5qKPeBfI7w8oqx2HapIsiDr9_-7Gdz5Hw1DJO9-e6S48jKCRjXsrP4J7vn0Me-MWCfPyhr1jIYwznI_vwffT2ZjNr9e_PdUTQpOyaSipw-Ynnwr2ecMmG8o3QrdjiFfZCTJ_zybLen1DIrls3vklO_IXF-xjLJ3S9cZ7AmfTybej4zRWT0gbUeguzY1vcmUtXrWW2iPuqI3NpM8cElMqxSebEslarewis42kTuVxipV0udBWPIVRu2r9c2A8U1ZjkzO1l8IbU3OhdIbYDm2iuE3gkOaxuuwFMiqSrA4Nq_WPKq6ASjtelg3ii4V0ckG4TDnkWo0y3mLTIoH3ZIWKFhZOtbMxPwAfgSSqqjHJ9kheFjKB_cFIXBBu2H1rxyouyE31130SeLPtpjspyKz1ONk0xoTMYhzzrDf79pWEIRHlPEtADxxi8M7DnvbXzyDXja5fcCXEi_8_10t4wBEw9cGF-zDq1tf-FQKern4dvfoPI6sAJg priority: 102 providerName: ProQuest – databaseName: Scholars Portal Open Access Journals dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Nj9MwELWW5cIFActHYEFGQnAKJLbj2AeEytKyIO0eKBW9Rbbj8qE2hTRF9N8z47irjZYDlx5sN008dvxeNfMeIc8EsDXgaDbNvLAp4H-ZqjznqcsdOorIurQhy_dcns7Ex3kxPyB7t9E4gZt_Ujv0k5q1y5d_fu3ewIZ_jYwTKPsrLFQHnqBQheAauc5QkguT-CLQ_xFxkQpebwzONKxHmfdZ8FcuELX7r76hLx1Rw_TJS-fR5Ba5GYEkHfWRv00OfHOHHI0aINGrHX1OQ2pn-M_8iHz5dD6i023726PHEISZToLNDp2evS_ohw0db7AGCZYiBQxLz9bbjafjlW13KJxLp51f0RO_XNJ30U6l6wN6l8wm488np2l0VEhrXqguzbWvc2kMfCollAcsYrXJhM8ckFW05xN1CQTOSrPITC2wU3rAUFK4nCvD75HDZt34B4SyTBoFTU5bL7jX2jIuVQZ4D3ipZCYhb3Eeq5-9aEaFMtahYd1-reKuqJRjZVkD5lgIJxaI1aQD_lVL7Q00LRLyAqNQYfhhqp2JNQNwCyhbVY1QykewshAJOR6MhE3iht37OFb7NVbxUMOiuVYJeXrRjd_ExLPGw2TjGB2qjWHM_T7sF4_ENQor51lC1GBBDJ552NN8_xYkvGE7FExy_vA_fvgRucEASfVZh8fksGu3_jEgoc4-CYv8L8j3BUk priority: 102 providerName: Scholars Portal |
| Title | RNA Surveillance Factor SMG5 Is Essential for Mouse Embryonic Stem Cell Differentiation |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/39199410 https://www.proquest.com/docview/3097839398 https://www.proquest.com/docview/3099142268 https://pubmed.ncbi.nlm.nih.gov/PMC11352633 https://doaj.org/article/8c277d512f4c4f83926c966d69eaf4cf |
| Volume | 14 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9NAEB215cIFAeXDUKJFQnByY3vX691jGhIKUqKqoSI3y7veQFHsVomL1H_PzNqpYnHjsof9sOydWfs9680MwAeBbA05mgkjJ0yI-F-GKo55aGNLFUVkmRmv8p3L8yvxbZkuD0DuYmG8aN-a69N6XZ3W17-8tvK2ssOdTmx4MRvjxdJEcj48hEP00D2O_rvDQCrhrcqdI6cfUiQ7EglFaQq63Pz_voH3PkF9eeTe92b6FJ50QJGN2ht6Bgeufg7HoxpJcnXPPjIv3fT_xI_hx-V8xBZ3mz-OagihGdnUl9Fhi9mXlH3dssmWYozQ1RhiVDZDtu_YpDKbe0qMyxaNq9jYrdfsc1cupWkN9gKuppPv4_Owq5gQljxVTRhrV8ayKLBVSiiHWMPoIhIuskhGqfyeKDMkaEYWq6goBQ1KhxhJChtzVfCXcFTf1O41sCSShcIuq40T3GltEi5VhHgOeadMigDOaB_z2zYpRk5pqn3HzeZn3hkrVzbJshIxxUpYsSIsJi3yq1JqV2DXKoBPZIWcDhNutS26mAC8BUpLlY8oVY9IslQEcNKbiYfA9od3dsy7Q7jNuY9R0VyrAN4_DNNKEpbVDjeb5mgfTYxzXrVmf3gkrilxchwFoHoO0Xvm_gh6rE_RvfPQN_-_9C08ThBAtWLDEzhqNnfuHQKgxgzQ65fZAB6dTeYXlwP_GwHbmVADfxL-AsSQCos |
| link.rule.ids | 230,315,730,783,787,867,888,2109,12070,21402,24332,27938,27939,31733,31734,33758,33759,43324,43819,53806,53808,74081,74638 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Bb9MwFLZgHOCCBgMWNsBICE7Rkthx7BMqo6WDtQe6id4s23EBaU1HmyHt3_Oe45VFSFxysB0p8Xt2vs9573uEvOHA1oCj2TTz3KaA_0Uq85ylLndYUUTUlQ1RvlMxPuef5-U8HrhtYljlzZ4YNup65fCM_IiFhAPFlHx_-SvFqlH4dzWW0LhL7qEOF2rnV_Nqe8aCaEgWrIt3Z8DujzCnHSiFRMGCqNL_715862PUD5S89eUZ7ZKHETLSQWfjR-SObx6TvUEDdHl5Td_SEMQZTsf3yLev0wGdXa1_e6wmBAalo1BQh84mn0p6sqHDDWYbgdNRQKt0Arzf0-HSrq9RIpfOWr-kx_7ign6MhVPaznRPyPloeHY8TmPthLRmpWzTXPk6F8bAVUouPaAOq0zGfeaAlmIhPl5XQNWsMIvM1Bw7hYcJFtzlTBr2lOw0q8bvE1pkwkhocsp6zrxStmBCZoDswCKiMAn5gPOoLzt5DI2C1aFhtf6uo_9r6YqqqgFdLLjjC0RlwgHTqoXyBpoWCXmHVtC4rGCqnYnZAfAIKFClByjaw4uq5Ak57I2E5eD63Td21HE5bvRf50nI62033okhZo2HycYxKuQVw5hnndm3r8QUSijnWUJkzyF679zvaX7-CGLd4PhlIRh7_v_nekXuj88mp_r0ZPrlgDwoADp1YYaHZKddX_kXAH1a-zL49x8GJgGx |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Rb9MwELZgSIgXBAxYxgAjIXiKmsSOYz-hsrVswCpEmehbZDsOIK3p1qRI-_fcOV5ZhMRLHmxHcnx39nfO3X2EvObgrYGPZuLEcRMD_hexTFMW29Qio4ioCuOjfGfi-Ix_XOSLEP_UhrDK6z3Rb9TVyuId-Yj5hAPFlBzVISziy9H03cVljAxS-Kc10GncJncKDgcd6HaxKLb3LYiMZMb62HcGnv4I89tTHJkgV5Gv2P_vvnzjYBoGTd44haYPyP0AH-m4l_dDcss1j8juuAHXeXlF31Af0OlvynfJ96-zMZ1v1r8dMguBcOnUk-vQ-emHnJ60dNJi5hEoIAXkSk9Xm9bRydKsr7BcLp13bkkP3fk5PQokKl0vxsfkbDr5dngcBx6FuGK57OJUuSoVWsNTSi4dIBCjdMJdYsFFRVI-XhXgthmh60RXHDuFg8UW3KZMavaE7DSrxu0RmiVCS2iyyjjOnFImY0ImgPJAOiLTEXmP61he9KUySixe7RtW6x9lsIVS2qwoKkAaNbe8RoQmLHhdlVBOQ1MdkbcohRJNDJba6pApAFPAYlXlGAv48KzIeUQOBiPBNOyw-1qOZTDNtvyrSBF5te3GNzHcrHGw2DhG-RxjGPO0F_v2k5jCcsppEhE5UIjBNw97ml8_feFuMII8E4zt_39eL8ldUO3y88ns0zNyLwMU1UccHpCdbr1xzwEFdeaFV-8_GPkF5g |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=RNA+Surveillance+Factor+SMG5+Is+Essential+for+Mouse+Embryonic+Stem+Cell+Differentiation&rft.jtitle=Biomolecules+%28Basel%2C+Switzerland%29&rft.au=Chen%2C+Chengyan&rft.au=Wei%2C+Yanling&rft.au=Jiang%2C+Xiaoning&rft.au=Li%2C+Tangliang&rft.date=2024-08-01&rft.issn=2218-273X&rft.eissn=2218-273X&rft.volume=14&rft.issue=8&rft_id=info:doi/10.3390%2Fbiom14081023&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2218-273X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2218-273X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2218-273X&client=summon |