Doxycycline-Mediated Control of Cyclin D2 Overexpression in Human-Induced Pluripotent Stem Cells

• Published in: International journal of molecular sciences Vol. 25; no. 16; p. 8714
• Main Authors: Qiao, Aijun, Wei, Yuhua, Liu, Yanwen, Kahn-Krell, Asher, Ye, Lei, Nguyen, Thanh, Zhang, Jianyi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.08.2024; MDPI
• Subjects: Cell Differentiation - drug effects; Cloning; Communication; cyclin D2; Cyclin D2 - genetics; Cyclin D2 - metabolism; Doxycycline - pharmacology; doxycycline-inducible Tet-On transactivator; Gene Expression Regulation - drug effects; Genomes; human-induced pluripotent stem cells; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - drug effects; Induced Pluripotent Stem Cells - metabolism; myocardial regeneration; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Plasmids; Promoter Regions, Genetic; Proteins; RNA, Guide, CRISPR-Cas Systems; Stem cells; United States > US; cyclin D2; human-induced pluripotent stem cells; doxycycline-inducible Tet-On transactivator; myocardial regeneration

Previous studies have demonstrated that when the cyclin D2 (CCND2), a cell-cycle regulatory protein, is overexpressed in human-induced pluripotent stem cells (hiPSCs), cardiomyocytes (CMs) differentiated from these CCND2-overexpressing hiPSCs can proliferate after transplantation into infarcted hear...