The UPLC-ESI-QqQLIT-MS/MS method for quantitative determination of phytochemicals in ethanolic extracts of different parts of eight Ficus species: Development and validation
Ficus and validation of the ultra performance liquid chromatography-electrospray ionization hybrid triple quadrupole-linear ion trap-tandem mass spectrometry (UPLC-ESI-QqQ LIT -MS/MS) method in a multiple-reaction monitoring (MRM) mode for the quantitative determination of 19 phytochemicals. The chr...
Saved in:
Published in | International journal of food properties Vol. 21; no. 1; pp. 328 - 344 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Abingdon
Taylor & Francis
01.01.2018
Taylor & Francis Ltd Taylor & Francis Group |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Ficus and validation of the ultra performance liquid chromatography-electrospray ionization hybrid triple quadrupole-linear ion trap-tandem mass spectrometry (UPLC-ESI-QqQ
LIT
-MS/MS) method in a multiple-reaction monitoring (MRM) mode for the quantitative determination of 19 phytochemicals. The chromatographic separation of targeted phytochemicals was performed using the Waters ACQUITY UPLC BEH™ C18 column (1.7 μm, 2.1 mm × 50 mm) with 0.1% formic acid with water and acetonitrile as a mobile phase at a flow rate of 0.25 mL/min. The validation parameters showed the overall recoveries from 95.78−101.44% (RSD ≤ 3.25%), precision (intra-day: RSD ≤ 2.96%; inter-day: RSD ≤ 2.89%), linearity (R
2
≥ 0.9982), limit of detection (8.60 × 10
-10
−2.18 × 10
-6
mg/mL), and the limit of quantitation (2.60 × 10
-9
-6.63 × 10
-6
mg/mL) in the concentration range from 0.5 to 1000 × 10
-6
mg/mL. This method was successfully applied in ethanolic extracts of different parts (fruits, leaves, and barks) of selected eight Ficus species. Quinic acid was predominant followed by rutin and chlorogenic acid among the studied nineteen phytochemicals. Ficus benjamina showed the maximum total content in fruits and leaves. The UPLC-ESI-QqQ
LIT
-MS/MS method combined with principal component analysis (PCA) was successfully used for Ficus species discrimination on the basis of the contents of 15 compounds. The UPLC-ESI-QqQ
LIT
-MS/MS method combined with PCA could be used for quality control. |
---|---|
AbstractList | Ficus and validation of the ultra performance liquid chromatography–electrospray ionization hybrid triple quadrupole–linear ion trap–tandem mass spectrometry (UPLC–ESI–QqQLIT–MS/MS) method in a multiple-reaction monitoring (MRM) mode for the quantitative determination of 19 phytochemicals. The chromatographic separation of targeted phytochemicals was performed using the Waters ACQUITY UPLC BEH™ C18 column (1.7 μm, 2.1 mm × 50 mm) with 0.1% formic acid with water and acetonitrile as a mobile phase at a flow rate of 0.25 mL/min. The validation parameters showed the overall recoveries from 95.78−101.44% (RSD ≤ 3.25%), precision (intra-day: RSD ≤ 2.96%; inter-day: RSD ≤ 2.89%), linearity (R2 ≥ 0.9982), limit of detection (8.60 × 10–10−2.18 × 10–6 mg/mL), and the limit of quantitation (2.60 × 10–9–6.63 × 10–6 mg/mL) in the concentration range from 0.5 to 1000 × 10–6 mg/mL. This method was successfully applied in ethanolic extracts of different parts (fruits, leaves, and barks) of selected eight Ficus species. Quinic acid was predominant followed by rutin and chlorogenic acid among the studied nineteen phytochemicals. Ficus benjamina showed the maximum total content in fruits and leaves. The UPLC–ESI–QqQLIT–MS/MS method combined with principal component analysis (PCA) was successfully used for Ficus species discrimination on the basis of the contents of 15 compounds. The UPLC–ESI–QqQLIT–MS/MS method combined with PCA could be used for quality control. Ficus and validation of the ultra performance liquid chromatography–electrospray ionization hybrid triple quadrupole–linear ion trap–tandem mass spectrometry (UPLC–ESI–QqQLIT–MS/MS) method in a multiple-reaction monitoring (MRM) mode for the quantitative determination of 19 phytochemicals. The chromatographic separation of targeted phytochemicals was performed using the Waters ACQUITY UPLC BEH™ C18 column (1.7 μm, 2.1 mm × 50 mm) with 0.1% formic acid with water and acetonitrile as a mobile phase at a flow rate of 0.25 mL/min. The validation parameters showed the overall recoveries from 95.78−101.44% (RSD ≤ 3.25%), precision (intra-day: RSD ≤ 2.96%; inter-day: RSD ≤ 2.89%), linearity (R2 ≥ 0.9982), limit of detection (8.60 × 10–10−2.18 × 10–6 mg/mL), and the limit of quantitation (2.60 × 10–9–6.63 × 10–6 mg/mL) in the concentration range from 0.5 to 1000 × 10–6 mg/mL. This method was successfully applied in ethanolic extracts of different parts (fruits, leaves, and barks) of selected eight Ficus species. Quinic acid was predominant followed by rutin and chlorogenic acid among the studied nineteen phytochemicals. Ficus benjamina showed the maximum total content in fruits and leaves. The UPLC–ESI–QqQLIT–MS/MS method combined with principal component analysis (PCA) was successfully used for Ficus species discrimination on the basis of the contents of 15 compounds. The UPLC–ESI–QqQLIT–MS/MS method combined with PCA could be used for quality control. Ficus and validation of the ultra performance liquid chromatography-electrospray ionization hybrid triple quadrupole-linear ion trap-tandem mass spectrometry (UPLC-ESI-QqQ LIT -MS/MS) method in a multiple-reaction monitoring (MRM) mode for the quantitative determination of 19 phytochemicals. The chromatographic separation of targeted phytochemicals was performed using the Waters ACQUITY UPLC BEH™ C18 column (1.7 μm, 2.1 mm × 50 mm) with 0.1% formic acid with water and acetonitrile as a mobile phase at a flow rate of 0.25 mL/min. The validation parameters showed the overall recoveries from 95.78−101.44% (RSD ≤ 3.25%), precision (intra-day: RSD ≤ 2.96%; inter-day: RSD ≤ 2.89%), linearity (R 2 ≥ 0.9982), limit of detection (8.60 × 10 -10 −2.18 × 10 -6 mg/mL), and the limit of quantitation (2.60 × 10 -9 -6.63 × 10 -6 mg/mL) in the concentration range from 0.5 to 1000 × 10 -6 mg/mL. This method was successfully applied in ethanolic extracts of different parts (fruits, leaves, and barks) of selected eight Ficus species. Quinic acid was predominant followed by rutin and chlorogenic acid among the studied nineteen phytochemicals. Ficus benjamina showed the maximum total content in fruits and leaves. The UPLC-ESI-QqQ LIT -MS/MS method combined with principal component analysis (PCA) was successfully used for Ficus species discrimination on the basis of the contents of 15 compounds. The UPLC-ESI-QqQ LIT -MS/MS method combined with PCA could be used for quality control. |
Author | Kumar, Brijesh Kushwaha, Arun K. Kumar, Sunil Singh, Awantika Tiwari, Rinkey Chaudhary, Lal B. Srivastava, Mukesh |
Author_xml | – sequence: 1 givenname: Sunil surname: Kumar fullname: Kumar, Sunil organization: Sophisticated Analytical Instrument Facility (SAIF), CSIR-Central Drug Research Institute – sequence: 2 givenname: Awantika surname: Singh fullname: Singh, Awantika organization: Academy of Scientific and Innovative Research (AcSIR) – sequence: 3 givenname: Arun K. surname: Kushwaha fullname: Kushwaha, Arun K. organization: Plant Diversity, Systematics and Herbarium Division, CSIR-National Botanical Research Institute – sequence: 4 givenname: Rinkey surname: Tiwari fullname: Tiwari, Rinkey organization: Plant Diversity, Systematics and Herbarium Division, CSIR-National Botanical Research Institute – sequence: 5 givenname: Lal B. surname: Chaudhary fullname: Chaudhary, Lal B. organization: Plant Diversity, Systematics and Herbarium Division, CSIR-National Botanical Research Institute – sequence: 6 givenname: Mukesh surname: Srivastava fullname: Srivastava, Mukesh organization: Biometry and Statistics Division, CSIR-Central Drug Research Institute – sequence: 7 givenname: Brijesh surname: Kumar fullname: Kumar, Brijesh email: brijesh_kumar@cdri.res.in organization: Academy of Scientific and Innovative Research (AcSIR) |
BookMark | eNo1kd1uEzEQhVeoSLSFR0CyxPWm_sv-cAUKLURKVaqk19asPds42rU3XieQh-Id8Tbtle3R53Nm5lxlF847zLLPjM4YregNo7XkNeMzTlk5Y5LJSlTvsks2Fzznoiou0j0x-QR9yK7GcUcpqwSjl9m_zRbJ0-_VIr9dL_PH_eNqucnv1zf3a9Jj3HpDWh_I_gAu2gjRHpEYjBh669LLO-JbMmxP0est9lZDNxLrSPoJzndWE_wbA-g4TpyxbYsBXSQDhHMJ7fM2kjurDyMZB9QWx6_kBx6x80M_keAMOUJnzYvbx-x9myzw0-t5nT3d3W4Wv_LVw8_l4vsqN7yoqhwbEKWp-bwWtTEFLY2kouG6ZkyilC3FFiQXoBtRoihpOZclAOrSaGqawojrbHnWNR52agi2h3BSHqx6KfjwrNIEVneoIC1YVrJueKHlZKmxqWuYF2VDsZhPWl_OWkPw-wOOUe38IbjUvuKslIwzTmmivp0p69LCe_jjQ2dUhFPnQxvAaTuqFJiaAldvgaspcPUauPgPBDuiHQ |
ContentType | Journal Article |
Copyright | 2018 The Author(s). Published by Taylor & Francis. 2018 2018 The Author(s). Published by Taylor & Francis. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
Copyright_xml | – notice: 2018 The Author(s). Published by Taylor & Francis. 2018 – notice: 2018 The Author(s). Published by Taylor & Francis. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
DBID | 0YH 7QR 8FD FR3 P64 DOA |
DOI | 10.1080/10942912.2017.1414838 |
DatabaseName | Taylor & Francis Open Access Chemoreception Abstracts Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts Directory of Open Access Journals |
DatabaseTitle | Chemoreception Abstracts Engineering Research Database Technology Research Database Biotechnology and BioEngineering Abstracts |
DatabaseTitleList | Chemoreception Abstracts |
Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: 0YH name: Taylor & Francis Open Access url: https://www.tandfonline.com sourceTypes: Publisher |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Diet & Clinical Nutrition |
EISSN | 1532-2386 |
EndPage | 344 |
ExternalDocumentID | oai_doaj_org_article_a2384849b26c49259ceb99a567b0e65d 1414838 |
Genre | Original Articles |
GroupedDBID | .7F .QJ 0R~ 0YH 29J 4.4 53G 5GY 5VS A8Z AAAVI AAENE ABCCY ABDBF ABFIM ABHAV ABPEM ABPTK ABTAI ACGEJ ACGFS ACIWK ACPRK ADBBV ADCVX ADXPE AENEX AEPSL AFKVX AGMYJ AHDLD AIJEM AJWEG ALMA_UNASSIGNED_HOLDINGS AVBZW AWYRJ BCNDV CE4 CS3 DGEBU DU5 EAP EBO EBS ECGQY EHX EJD EMK EPL ESX E~A E~B FUNRP FVPDL GROUPED_DOAJ GTTXZ H13 HF~ HZ~ H~P I-F IPNFZ J.P M4Z NA5 O9- OK1 P2P PQEST PQQKQ RIG S-T TEI TFL TFT TFW TH9 TUS TWF UCJ UT5 UU3 V1K ~S~ 7QR 8FD AAHBH ESTFP FR3 P64 TDBHL |
ID | FETCH-LOGICAL-d2688-eba37d925939dd607d403b2c9114e44f0efa423acb37e3707547aaec7dc0db6d3 |
IEDL.DBID | 0YH |
ISSN | 1094-2912 |
IngestDate | Thu Sep 05 15:30:02 EDT 2024 Fri Sep 13 04:15:57 EDT 2024 Tue Jul 04 18:17:44 EDT 2023 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Language | English |
License | open-access: http://creativecommons.org/licenses/by/4.0/: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-d2688-eba37d925939dd607d403b2c9114e44f0efa423acb37e3707547aaec7dc0db6d3 |
OpenAccessLink | https://www.tandfonline.com/doi/abs/10.1080/10942912.2017.1414838 |
PQID | 2174121200 |
PQPubID | 176142 |
PageCount | 17 |
ParticipantIDs | doaj_primary_oai_doaj_org_article_a2384849b26c49259ceb99a567b0e65d proquest_journals_2174121200 informaworld_taylorfrancis_310_1080_10942912_2017_1414838 |
PublicationCentury | 2000 |
PublicationDate | 1/1/2018 20180101 2018-01-01 |
PublicationDateYYYYMMDD | 2018-01-01 |
PublicationDate_xml | – month: 01 year: 2018 text: 1/1/2018 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | Abingdon |
PublicationPlace_xml | – name: Abingdon |
PublicationTitle | International journal of food properties |
PublicationYear | 2018 |
Publisher | Taylor & Francis Taylor & Francis Ltd Taylor & Francis Group |
Publisher_xml | – name: Taylor & Francis – name: Taylor & Francis Ltd – name: Taylor & Francis Group |
SSID | ssj0018310 |
Score | 2.196554 |
Snippet | Ficus and validation of the ultra performance liquid chromatography-electrospray ionization hybrid triple quadrupole-linear ion trap-tandem mass spectrometry... Ficus and validation of the ultra performance liquid chromatography–electrospray ionization hybrid triple quadrupole–linear ion trap–tandem mass spectrometry... |
SourceID | doaj proquest informaworld |
SourceType | Open Website Aggregation Database Publisher |
StartPage | 328 |
SubjectTerms | Acetonitrile Bioactive compounds Chlorogenic acid Ficus Ficus species Formic acid Fruits Ionization Ions Leaves Liquid chromatography LIT Mass spectroscopy MS/MS Phytochemicals Principal component analysis Quality control Quantitation Quinic acid Rutin Species UPLC-ESI-QqQ UPLC–ESI–QqQLIT–MS/MS |
SummonAdditionalLinks | – databaseName: Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3NatwwEBYlp15Kf-m2aZhD6M2sLMvWqrc0yZKUbGjYLOQm9AsLxflZ773vkEfpG_VJOiPbsDSHXnoxRhhLaD6NZkaabxg7LBUFN1QsYvChkKEsC5tEomw0n9xMlNZSovDisjlbyW839c1OqS-6E9bTA_cTN7W4p8iZ1E40noj0tI9Oa1s3yvHY1CFr37Ienanh_IDKZ-VzTi0LoUsx5u7M-JTaqImudSlUFOgPUHJKZuz_i6_0iX7Om878JXsxWItw1I_yFXsW29dscrKOHXyGgdLzB1yOjPpv2C8UO6y-Xxz__vl4ujzH59X91cX5Nb4sltPFEvqK0YC9w_3WtjnFDBUehPFaDP0HbhPg9HdUTSvTCWxg3UKkKDvRCAMqdEqu2tB3Y4WVDu5wMnNTjrbCfO23G6BMTnTGv8DO7SSwbQBE-Lqv5_SWrean18dnxVCXoQiiwYUVna1UIFlUOoSGqyB55YRHvSmjlInHZNFKs95VKlYKjRKpUOReBc-Da0L1ju21t218z8DVScq6RsCgHHAPsI5bIZJS2lPyip6wryQXc9dTbxgiw84NCBEzQMT8CyITpnelarocC0l94RJTlQNL6ggMQ8AwAzAmbH-EgBlW98aQG1fins_5h_8xvo_sOXY56wM7-2yve9jGT2jqdO4go_oPJCL9eQ priority: 102 providerName: Directory of Open Access Journals |
Title | The UPLC-ESI-QqQLIT-MS/MS method for quantitative determination of phytochemicals in ethanolic extracts of different parts of eight Ficus species: Development and validation |
URI | https://www.tandfonline.com/doi/abs/10.1080/10942912.2017.1414838 https://www.proquest.com/docview/2174121200/abstract/ https://doaj.org/article/a2384849b26c49259ceb99a567b0e65d |
Volume | 21 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELagvXBBPMVCWc0BcYvIw4nX3LptV1vUrai2K8HJ8hOtVGXbJvuz-I_MOA6qQJw4RbESR8o8PB7P9w1jHwpByQ3hM--sy7grikyHMhAazQYzKwutCSi8umyWG_7lWz1WE3aprJL20GEgioi-moxbm26siMOrRC9aEIyqEGjqGNFXs8fssMTQm6r68u_L3wcJ1EcrHnhKntE7I4jnX9Mk6v4_iEv_ctRx9Vk8Y09T2AjHg5yfs0e-fcEmp1vfw0dI3J43cDlS679kP1H-sPl6cZKdrc-zq7uri_PrbLX-tFrD0DQa8Ltwt9dtRJmhzwM3VsbQDLALgBLoqaFWZBToYNuCp0Q7MQkD-nTCV3X03NhkpYdbVMQ4FBOusNjafQcE5sT9-Gd4UKAE-PMBlXw7tHR6xTaLs-uTZZZaM2SubNC2vNGVcBL3TpV0rsmF43llSouuk3vOQ-6DxkBNW1MJXwmMS7hAqVvhbO5M46rX7KDdtf4NA1MHzusadQYlgMuANrkuyyCEtIRfkRM2J4mo24F9QxEfdhzY3f9QybyUxsiDz7g0ZWOJblFab6TUdSNM7pvaTZh8KE_Vx3RIGHqXqKpIRKmjSihSCZVUYsKORuGrZOCdop1cgct-nr_9j6nfsSd4OxtSOkfsoL_f-_cY5PRmGtV4yg6P56fzxTSmCn4BS3r33g |
link.rule.ids | 315,786,790,870,2115,27535,27957,27958,59498,59499 |
linkProvider | Taylor & Francis |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lj9MwELZgOcAF8RSFBeaAuFnk4cQ1N1i2aqGtWLWVlpPlJ6qE0t1t-rP4j8w4CapAnDhFysORMt-MxxN_3zD2JpdU3JCBB-88Fz7PuYlFJDaai3Zc5MYQUXixrKcb8fmyujziwtC2SlpDx04oIsVqcm4qRg9b4vCoMIzmxKPKJfo6pvTl-Da7U5HSCmI6-zb9_SeBGmmlP55KcHpmYPH8a5heu_8P5dK_InWafiYP2P0-b4QPnaEfsluhecRGn7ahhbfQi3v-gOWgrf-Y_UQAwObr_Iyfr2b84vpiPlvzxerdYgVd12jA98L1wTSJZoZBD_ywNYZGgF0ENEFLHbWSpMAetg0EqrSTlDBgUCeC1Z7uG7qstHCFSEynUsUVJlt32AOxOXFB_h6OdigBfn1AlG-7nk5P2GZyvj6b8r43A_dFjc4VrCmlV7h4KpX3dSa9yEpbOIydIggRsxANZmrG2VKGUmJiIiSa3UnvMm9rXz5lJ82uCc8Y2CoKUVUIGrQAzgPGZqYoopTKEYFFjdhHsoi-6uQ3NAlipxO7m--69y9tMPUQY6FsUTvSW1QuWKVMVUubhbryI6aO7anbVA-JXfMSXea9UuoACU2Q0D0kRux0ML7uPXyvaSmX47yfZc__Y-jX7O50vZjr-Wz55QW7h5fGXX3nlJ20N4fwEjOe1r5KkP4FLrv47Q |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9NAEF5BkRAXRHmIQIE5IG4Wfqy9WW6lbZRAErVKI8Fptc8qEnLS2vlZ_MfOrG1UgXriZGltryV_M7OzszPfMPYxExTcED7xzrqEuyxLdMgDVaPZYMZ5pjUVCi-W1XTNv_0oh2zCpk-rpD106Igioq0m5d65MGTE4VWiFc2ojCoTqOro0Rfjh-xRKbmkrK705_TPQQL10YoHnpIn9M5QxHPfND11_1_Epf8Y6rj6TJ6xp73bCMcdzofsga-fs9HpxrfwCXpuz1-wHKj1X7DfiD-sz-cnydlqllxcX8xnl8li9Xmxgq5pNOB34Xqv61hlhjYP3JAZQzPANgAi0FJDrcgo0MCmBk-BdmISBrTpVF_V0HNDk5UWdiiIcSgGXGGysfsGqJgT9-Nf4E6CEuDPBxTyTdfS6SVbT84uT6ZJ35ohcXmFuuWNLoSTuHcqpHNVKhxPC5NbNJ3ccx5SHzQ6atqaQvhCoF_CBaJuhbOpM5UrXrGDelv71wxMGTgvS5QZRACXAW1SnedBCGmpfkWO2FdCRO069g1FfNhxYHtzpXr1Uho9Dz7m0uSVJbpFab2RUpeVMKmvSjdi8i6eqo3hkND1LlFF1hOlDiKhSCRULxIjdjSAr3oFbxTt5DJc9tP0zX9M_YE9Pj-dqPls-f0te4J3xl1054gdtDd7_w79nda8jxJ9C6Ue-BY |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+UPLC-ESI-QqQLIT-MS%2FMS+method+for+quantitative+determination+of+phytochemicals+in+ethanolic+extracts+of+different+parts+of+eight+Ficus+species%3A+Development+and+validation&rft.jtitle=International+journal+of+food+properties&rft.au=Kumar%2C+Sunil&rft.au=Singh%2C+Awantika&rft.au=Kushwaha%2C+Arun+K.&rft.au=Tiwari%2C+Rinkey&rft.date=2018-01-01&rft.pub=Taylor+%26+Francis&rft.issn=1094-2912&rft.eissn=1532-2386&rft.volume=21&rft.issue=1&rft.spage=328&rft.epage=344&rft_id=info:doi/10.1080%2F10942912.2017.1414838&rft.externalDBID=0YH&rft.externalDocID=1414838 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1094-2912&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1094-2912&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1094-2912&client=summon |