Lipopolysaccharide Core Truncation in Invasive Escherichia coli O157:H7 ATCC 43895 Impairs Flagella and Curli Biosynthesis and Reduces Cell Invasion Ability

• Published in: International journal of molecular sciences Vol. 25; no. 17; p. 9224
• Main Authors: Sheng, Haiqing, Ndeddy Aka, Robinson J, Wu, Sarah
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.09.2024
• Subjects: Animals; Antigens; Bacterial Adhesion; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biofilms; Biofilms - growth & development; Biosynthesis; bovine host; Cattle; Cell growth; cell invasion; E coli; Epithelial Cells - metabolism; Epithelial Cells - microbiology; Escherichia coli O157 - genetics; Escherichia coli O157 - metabolism; Escherichia coli O157 - physiology; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Flagella - genetics; Flagella - metabolism; invasive E. coli O157:H7; Lipids; Lipopolysaccharides - biosynthesis; LPS core; Motility; Mutation; Pathogens; production of flagella and curli; Virulence; cell invasion; bovine host; production of flagella and curli; invasive E. coli O157:H7; LPS core
• DOI: 10.3390/ijms25179224
• ISSN: 1661-6596; 1422-0067

O157:H7 ( O157) is known for causing severe foodborne illnesses such as hemorrhagic colitis and hemolytic uremic syndrome. Although O157 is typically regarded as an extracellular pathogen and a weak biofilm producer, some O157 strains, including a clinical strain ATCC 43895, exhibit a notable ability to invade bovine crypt cells and other epithelial cells, as well as to form robust biofilm. This invasive strain persists in the bovine host significantly longer than non-invasive strains. Various surface-associated factors, including lipopolysaccharides (LPS), flagella, and other adhesins, likely contribute to this enhanced invasiveness and biofilm formation. In this study, we constructed a series of LPS-core deletion mutations ( , , , and ) in O157 ATCC 43895, resulting in stepwise truncations of the LPS. This approach enabled us to investigate the effects on the biosynthesis of key surface factors, such as flagella and curli, and the ability of this invasive strain to invade host cells. We confirmed the LPS structure and found that all LPS-core mutants failed to form biofilms, highlighting the crucial role of core oligosaccharides in biofilm formation. Additionally, the LPS inner-core mutants Δ and Δ lost the ability to produce flagella and curli. Furthermore, these inner-core mutants exhibited a dramatic reduction in adherence to and invasion of epithelial cells (MAC-T), showing an approximately 100-fold decrease in cell invasion compared with the outer-core mutants ( and ) and the wild type. These findings underscore the critical role of LPS-core truncation in impairing flagella and curli biosynthesis, thereby reducing the invasion capability of O157 ATCC 43895.