Lipopolysaccharide Core Truncation in Invasive Escherichia coli O157:H7 ATCC 43895 Impairs Flagella and Curli Biosynthesis and Reduces Cell Invasion Ability

O157:H7 ( O157) is known for causing severe foodborne illnesses such as hemorrhagic colitis and hemolytic uremic syndrome. Although O157 is typically regarded as an extracellular pathogen and a weak biofilm producer, some O157 strains, including a clinical strain ATCC 43895, exhibit a notable abilit...

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Published inInternational journal of molecular sciences Vol. 25; no. 17; p. 9224
Main Authors Sheng, Haiqing, Ndeddy Aka, Robinson J, Wu, Sarah
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.09.2024
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Summary:O157:H7 ( O157) is known for causing severe foodborne illnesses such as hemorrhagic colitis and hemolytic uremic syndrome. Although O157 is typically regarded as an extracellular pathogen and a weak biofilm producer, some O157 strains, including a clinical strain ATCC 43895, exhibit a notable ability to invade bovine crypt cells and other epithelial cells, as well as to form robust biofilm. This invasive strain persists in the bovine host significantly longer than non-invasive strains. Various surface-associated factors, including lipopolysaccharides (LPS), flagella, and other adhesins, likely contribute to this enhanced invasiveness and biofilm formation. In this study, we constructed a series of LPS-core deletion mutations ( , , , and ) in O157 ATCC 43895, resulting in stepwise truncations of the LPS. This approach enabled us to investigate the effects on the biosynthesis of key surface factors, such as flagella and curli, and the ability of this invasive strain to invade host cells. We confirmed the LPS structure and found that all LPS-core mutants failed to form biofilms, highlighting the crucial role of core oligosaccharides in biofilm formation. Additionally, the LPS inner-core mutants Δ and Δ lost the ability to produce flagella and curli. Furthermore, these inner-core mutants exhibited a dramatic reduction in adherence to and invasion of epithelial cells (MAC-T), showing an approximately 100-fold decrease in cell invasion compared with the outer-core mutants ( and ) and the wild type. These findings underscore the critical role of LPS-core truncation in impairing flagella and curli biosynthesis, thereby reducing the invasion capability of O157 ATCC 43895.
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ISSN:1661-6596
1422-0067
1422-0067
DOI:10.3390/ijms25179224