Repetitive N-WASP-binding elements of the enterohemorrhagic Escherichia coli effector EspF(U) synergistically activate actin assembly

• Published in: PLoS pathogens Vol. 4; no. 10; p. e1000191
• Main Authors: Campellone, Kenneth G, Cheng, Hui-Chun, Robbins, Douglas, Siripala, Anosha D, McGhie, Emma J, Hayward, Richard D, Welch, Matthew D, Rosen, Michael K, Koronakis, Vassilis, Leong, John M
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science (PLoS) 01.10.2008
• Subjects: Actin Cytoskeleton - metabolism; Actin-Related Protein 2-3 Complex - metabolism; Amino Acid Sequence; Animals; Brain - metabolism; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cytoskeletal Proteins - genetics; Cytoskeletal Proteins - metabolism; DNA, Bacterial - genetics; DNA, Bacterial - metabolism; Enterohemorrhagic Escherichia coli - genetics; Enterohemorrhagic Escherichia coli - metabolism; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; GTP Phosphohydrolases - metabolism; Humans; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Molecular Sequence Data; Protein Structure, Tertiary; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Recombinant Fusion Proteins - metabolism; Repetitive Sequences, Amino Acid; Signal Transduction - genetics; Swine; Wiskott-Aldrich Syndrome Protein, Neuronal - metabolism

Enterohemorrhagic Escherichia coli (EHEC) generate F-actin-rich adhesion pedestals by delivering effector proteins into mammalian cells. These effectors include the translocated receptor Tir, along with EspF(U), a protein that associates indirectly with Tir and contains multiple peptide repeats that...