Defective flow space limits the scaling up of turbulence bioreactors for platelet generation

• Published in: Communications engineering Vol. 3; no. 1; pp. 77 - 12
• Main Authors: Okamoto, Haruki, Fujio, Kosuke, Nakamura, Sou, Harada, Yasuo, Hayashi, Hideki, Higashi, Natsumi, Ninomiya, Atsushi, Tanaka, Ryota, Sugimoto, Naoshi, Takayama, Naoya, Kaneda, Atsushi, Sawaguchi, Akira, Kato, Yoshikazu, Eto, Koji
• Format: Journal Article
• Language: English
• Published: London Springer Nature B.V 01.12.2024; Nature Publishing Group UK; Nature Portfolio
• Subjects: Antigens; Bioreactors; Clinical trials; Efficiency; Energy; Flow cytometry; Fluid dynamics; Impellers; Manufacturing; Scaling up; Shear stress; Simulation; Stem cells; Thrombocytopenia; Turbulence; Turbulent flow

To complement donor-dependent platelets supplies, we previously developed an ex vivo manufacturing system using induced pluripotent stem cell (iPSC)-derived expandable immortalized megakaryocyte progenitor cell lines (imMKCLs), and a turbulent flow bioreactor to generate iPSC-derived platelets produ...