Interação de proteínas Cry1 e Vip3A de Bacillus thuringiensis para controle de lepidópteros-praga

O objetivo deste trabalho foi avaliar a suscetibilidade das lagartas Anticarsia gemmatalis (Lepidoptera: Erebidae) e Chrysodeixis includens (Lepidoptera: Noctuidae) às proteínas Cry1 e Vip3A, bem como determinar se há a interação entre essas proteínas no controle das duas espécies. Bioensaios com as...

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Published inPesquisa agropecuaria brasileira Vol. 49; no. 2; pp. 79 - 87
Main Authors Crialesi-Legori, Paula Cristina Brunini, Davolos, Camila Chiaradia, Lemes, Ana Rita Nunes, Marucci, Suzana Cristina, Lemos, Manoel Victor Franco, Fernandes, Odair Aparecido, Desidério, Janete Apparecida
Format Journal Article
LanguageEnglish
Portuguese
Published Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira 01.02.2014
Embrapa Informação Tecnológica
Subjects
AGRICULTURE, DAIRY & ANIMAL SCIENCE
AGRICULTURE, MULTIDISCIPLINARY
Anticarsia gemmatalis
Chrysodeixis includens
manejo da resistência
piramidação de genes
sinergismo
soja transgênica
sinergismo
soja transgênica
Chrysodeixis includens
resistance management
transgenic soybean
Anticarsia gemmatalis
synergism
gene pyramiding
manejo da resistência
piramidação de genes
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Abstract O objetivo deste trabalho foi avaliar a suscetibilidade das lagartas Anticarsia gemmatalis (Lepidoptera: Erebidae) e Chrysodeixis includens (Lepidoptera: Noctuidae) às proteínas Cry1 e Vip3A, bem como determinar se há a interação entre essas proteínas no controle das duas espécies. Bioensaios com as proteínas isoladas e em combinações foram realizados, e as concentrações letais CL50 e CL90 foram estimadas para cada condição. As proteínas Cry1Aa, Cry1Ac e Vip3Af foram as mais efetivas no controle de A. gemmatalis, enquanto Cry1Ac, Vip3Aa e Vip3Af foram mais efetivas no de C. includens. As proteínas Cry1Ac e Cry1Ca causaram maior inibição do desenvolvimento das larvas sobreviventes à CL50, em ambas as espécies. Combinações entre Vip3A e Cry1 apresentam efeito sinérgico no controle das espécies e a combinação Vip3Aa+Cry1Ea destaca-se no controle de A. gemmatalis e C. includens. Essas proteínas combinadas são promissoras na construção de plantas piramidadas, para o controle simultâneo das pragas.
AbstractList O objetivo deste trabalho foi avaliar a suscetibilidade das lagartas Anticarsia gemmatalis (Lepidoptera: Erebidae) e Chrysodeixis includens (Lepidoptera: Noctuidae) às proteínas Cry1 e Vip3A, bem como determinar se há a interação entre essas proteínas no controle das duas espécies. Bioensaios com as proteínas isoladas e em combinações foram realizados, e as concentrações letais CL50 e CL90 foram estimadas para cada condição. As proteínas Cry1Aa, Cry1Ac e Vip3Af foram as mais efetivas no controle de A. gemmatalis, enquanto Cry1Ac, Vip3Aa e Vip3Af foram mais efetivas no de C. includens. As proteínas Cry1Ac e Cry1Ca causaram maior inibição do desenvolvimento das larvas sobreviventes à CL50, em ambas as espécies. Combinações entre Vip3A e Cry1 apresentam efeito sinérgico no controle das espécies e a combinação Vip3Aa+Cry1Ea destaca-se no controle de A. gemmatalis e C. includens. Essas proteínas combinadas são promissoras na construção de plantas piramidadas, para o controle simultâneo das pragas.
Author Fernandes, Odair Aparecido
Lemos, Manoel Victor Franco
Marucci, Suzana Cristina
Desidério, Janete Apparecida
Lemes, Ana Rita Nunes
Davolos, Camila Chiaradia
Crialesi-Legori, Paula Cristina Brunini
AuthorAffiliation Universidade Estadual Paulista
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  givenname: Paula Cristina Brunini
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  givenname: Camila Chiaradia
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DocumentTitleAlternate Interaction of Cry1 and Vip3A proteins of Bacillus thuringiensis for the control of lepidopteran insect pests
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Issue 2
Keywords sinergismo
soja transgênica
Chrysodeixis includens
resistance management
transgenic soybean
Anticarsia gemmatalis
synergism
gene pyramiding
manejo da resistência
piramidação de genes
Language English
Portuguese
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Snippet O objetivo deste trabalho foi avaliar a suscetibilidade das lagartas Anticarsia gemmatalis (Lepidoptera: Erebidae) e Chrysodeixis includens (Lepidoptera:...
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SubjectTerms AGRICULTURE, DAIRY & ANIMAL SCIENCE
AGRICULTURE, MULTIDISCIPLINARY
Anticarsia gemmatalis
Chrysodeixis includens
manejo da resistência
piramidação de genes
sinergismo
soja transgênica
Title Interação de proteínas Cry1 e Vip3A de Bacillus thuringiensis para controle de lepidópteros-praga
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