圈养东北虎猫冠状病毒的巢氏PCR检测

S852.65; 东北虎(Panthera tigris altaica)是全球生物多样性保护的旗舰物种,在维持生态系统功能中占据不可替代的重要地位.东北虎对于可感染家猫的病毒均易感,但目前关于东北虎病毒病的流行病学资料仍然十分有限.猫冠状病毒(Feline coronavi-rus,FCoV)感染,轻可导致猫科(Felidae)动物腹泻,重则呈现高致病性、致死性的猫传染性腹膜炎(Feline infectious peritonitis,FIP).因此,采用巢氏PCR方法对来自黑龙江省东北虎林园的6份东北虎全血样本进行FCoV检测,结果显示2份样本为阳性(H-11-1241、H-11-131...

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Published in野生动物学报 Vol. 45; no. 4; pp. 896 - 900
Main Authors 刘亚男, 谢薇, 徐海涛, 王阳平, 舒怡, 梁美玉, 王亚君
Format Journal Article
LanguageChinese
Published 东北林业大学野生动物与自然保护地学院,哈尔滨,150040%黑龙江东北虎林园,哈尔滨,150028 01.11.2024
Editorial Department of Chinese Journal of Wildlife
Subjects
东北虎
巢氏PCR
猫冠状病毒
巢氏PCR
Feline coronavirus
Nested PCR
猫冠状病毒
Amur tiger(Panthera ti-gris altaica)
东北虎
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Abstract S852.65; 东北虎(Panthera tigris altaica)是全球生物多样性保护的旗舰物种,在维持生态系统功能中占据不可替代的重要地位.东北虎对于可感染家猫的病毒均易感,但目前关于东北虎病毒病的流行病学资料仍然十分有限.猫冠状病毒(Feline coronavi-rus,FCoV)感染,轻可导致猫科(Felidae)动物腹泻,重则呈现高致病性、致死性的猫传染性腹膜炎(Feline infectious peritonitis,FIP).因此,采用巢氏PCR方法对来自黑龙江省东北虎林园的6份东北虎全血样本进行FCoV检测,结果显示2份样本为阳性(H-11-1241、H-11-1310),阳性率为33.3%.所得PCR片段与其他参考株同源性为99.3%～100.0%.研究结果丰富了圈养东北虎中猫冠状病毒流行病学数据,为其他圈养猫科动物的FCoV监测提供了参考,对野生猫科动物的保护与繁育具有重要意义.
AbstractList S852.65; 东北虎(Panthera tigris altaica)是全球生物多样性保护的旗舰物种,在维持生态系统功能中占据不可替代的重要地位.东北虎对于可感染家猫的病毒均易感,但目前关于东北虎病毒病的流行病学资料仍然十分有限.猫冠状病毒(Feline coronavi-rus,FCoV)感染,轻可导致猫科(Felidae)动物腹泻,重则呈现高致病性、致死性的猫传染性腹膜炎(Feline infectious peritonitis,FIP).因此,采用巢氏PCR方法对来自黑龙江省东北虎林园的6份东北虎全血样本进行FCoV检测,结果显示2份样本为阳性(H-11-1241、H-11-1310),阳性率为33.3%.所得PCR片段与其他参考株同源性为99.3%～100.0%.研究结果丰富了圈养东北虎中猫冠状病毒流行病学数据,为其他圈养猫科动物的FCoV监测提供了参考,对野生猫科动物的保护与繁育具有重要意义.
东北虎（Panthera tigris altaica）是全球生物多样性保护的旗舰物种，在维持生态系统功能中占据不可替代的重要地位。东北虎对于可感染家猫的病毒均易感，但目前关于东北虎病毒病的流行病学资料仍然十分有限。猫冠状病毒（Feline coronavirus，FCoV）感染，轻可导致猫科（Felidae）动物腹泻，重则呈现高致病性、致死性的猫传染性腹膜炎（Feline infectious peritonitis，FIP）。因此，采用巢氏PCR方法对来自黑龙江省东北虎林园的6份东北虎全血样本进行FCoV检测，结果显示2份样本为阳性（H-11-1241、H-11-1310），阳性率为33.3%。所得PCR片段与其他参考株同源性为99.3% ~ 100.0%。研究结果丰富了圈养东北虎中猫冠状病毒流行病学数据，为其他圈养猫科动物的FCoV监测提供了参考，对野生猫科动物的保护与繁育具有重要意义。
Abstract_FL Amur tiger(Panthera tigris altaica)is the flagship species for global biodiversity conservation.The tiger is sus-ceptible to the viruses that can infect domestic cats,but the epidemiological data on the virus diseases of Amur tiger are ex-ceedingly limited.Infection with Feline coronavirus(FCoV)usually causes general diarrhea in Felidae animals,while se-vere cases can present as highly pathogenic and lethal Feline infectious peritonitis(FIP).The nested PCR method was used to detect FCoV in six whole blood samples of Amur tiger from Siberian Tiger Park in Heilongjiang Province.The re-sults showed that two samples were positive(H-11-1241,H-11-1310),and the positive rate was 33.3%.The homology be-tween the obtained PCR fragments and other reference strains was 99.3%-100.0%.The results enrich the epidemiologi-cal data of FCoV in captive Amur tigers,provided reference for FCoV monitoring of other captive felines,and have great sig-nificance to the protection and breeding of wild felines.
Author 徐海涛
谢薇
梁美玉
王亚君
刘亚男
王阳平
舒怡
AuthorAffiliation 东北林业大学野生动物与自然保护地学院,哈尔滨,150040%黑龙江东北虎林园,哈尔滨,150028
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Author_FL XU Haitao
WANG Yajun
WANG Yangping
LIU Yanan
XIE Wei
LIANG Meiyu
SHU Yi
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Keywords 巢氏PCR
Feline coronavirus
Nested PCR
猫冠状病毒
Amur tiger(Panthera ti-gris altaica)
东北虎
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Snippet S852.65; 东北虎(Panthera tigris altaica)是全球生物多样性保护的旗舰物种,在维持生态系统功能中占据不可替代的重要地位.东北虎对于可感染家猫的病毒均易感,但目前关于东北虎病毒病的流行病学资料仍然十分有限.猫冠状病毒(Feline...
东北虎（Panthera tigris altaica）是全球生物多样性保护的旗舰物种，在维持生态系统功能中占据不可替代的重要地位。东北虎对于可感染家猫的病毒均易感，但目前关于东北虎病毒病的流行病学资料仍然十分有限。猫冠状病毒（Feline...
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SubjectTerms 东北虎
巢氏PCR
猫冠状病毒
Title 圈养东北虎猫冠状病毒的巢氏PCR检测
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